Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.
| Name | SKU | Size | Availability | Vendor | Price | Order | |
| Pluronic® F-127 | 59000 | 1 g | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing |
Pluronic® F-127 is a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading. Pluronic® F-127 is usually dissolved in water or DMSO with gentle heating. We also offer ready-to-use solutions of Pluronic® F-127, 20% in DMSO and Pluronic® F-127, 10% in H2O.
- White solid soluble in water and DMSO
- Store at room temperature
- MW: ~12,500
- [9003-11-6]
Pluronic F-127 is a registered trade mark of BASF.
REFERENCES
1) J. Membrane Biol. 19, 1 (1974).
2) Science 233, 886 (1986).
3) J. Biol Chem 262, 12801 (1987).
4) J. Biol. Chem. 265, 19543 (1990).
5) Methods Enzymol. 302, 341 (1999).
6) Methods Enzymol. 307, 441(1999).
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Pluronic® F-127 20% Solution in DMSO
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.
Log in for pricing -
Pluronic® F-127, 10% in H2O
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic compounds like AM esters of calcium indicator dyes to facilitate cell loading.
Log in for pricing -
Fura-2, AM Ester
Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.
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DMSO, Anhydrous
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
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