Donkey Anti-Chicken IgY (H+L), Highly Cross-Adsorbed

This is a highly cross-adsorbed donkey anti-chicken IgY (H L) secondary antibody labelled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum.

• Highly cross-adsorbed for specific staining with minimal background
• Available in 5 bright and photostable CF® dyes
• Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colours.

View Biotium’s full selection of Secondary Antibodies, or search our catalogue using Biotium’s Antibody Finder. Alternatively, you can view the secondary antibody product listings with catalogue numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see Biotium’s CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.