A fluorescent dsDNA quantitation solution with a wide linear range of 0.03-250 ng DNA. Designed for use in a fluorescence microplate reader.
| Name | SKU | Size | Availability | Vendor | Price | Order | |
AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution, Trial Size (200 assays) |
31027-T | 200 assays | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution (1000 assays) |
31027 | 1000 assays | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing |
Features
The AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified DNA samples, designed for use with fluorescence microplate readers. AccuClear® is our most popular DNA quantitation kit, with a very wide linear detection range from 0.03 – 250 ng. This solution does not come with a DNA standard; see the AccuClear® Ultra High Sensitivity dsDNA Quantitation Kits (31028 & 31029) which contains calf thymus DNA standards.
Features
- Biotium’s most popular & versatile DNA quantitation assay
- Extremely wide linear range of 0.03-250 ng DNA
- Highly specific for dsDNA
- Designed for fluorescence microplate readers
- Green fluorescence emission
Kit Components
- AccuClear® Dye, 100X
- DNA Quantitation Buffer, 20X Concentrate*
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
References
1. J Food Sci (2015) May;80(5):M1031-8 doi: 10.1111/1750-3841.12848
2. Analytical Biochemistry (2016) 15 Oct pp74-79 doi:10.1016/j.ab.2016.07.022
3. Microbiome (2018), Oct 20;6(1):188 doi:10.1186/s40168-018-0529-x
4. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102
Resources
Supporting Documents:
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