Uracil N-Glycosylase (UNG) Kit

Uracil N-Glycosylase (UNG) Kit

Cleave any DNA containing dUTP in place of dTTP

Key features

  • Hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine
  • Well-suited for use in DNA repair research studies
Name SKU Size Availability Vendor Price Order
Uracil N-Glycosylase (UNG) Kit UG131K 1,000 U Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Product Details

Kit contains Uracil N-Glycosylase (UNG) and UNG Storage Dilution Buffer.
 

Product Details

Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate. UNG is ideal for studying repair of abasic sites in double-stranded DNA.
 

Product specifications and usage

Unit Definition: One unit of UNG catalyzes the release of 1 nmol of uracil from uracil-containing DNA in 1 hour at 37°C under standard assay conditions.

Note: Epicentre’s unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme.

Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

Quality Control: UNG is free of detectable exo- and endonuclease and RNase activities.

You may also like

wp_reset_postdata();