High-fidelity, high-efficiency library amplification while maintaining even coverage
| Name | SKU | Size | Availability | Vendor | Price | Order | |
sparQ HiFi PCR Master Mix, 50 Rxns |
95192-050 | 50 Rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing | ||
sparQ HiFi PCR Master Mix, 250 Rxns |
95192-250 | 250 rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing |
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Features
- HiFi DNA polymerase engineered to minimize amplification bias
- Increased amplification efficiency resulting in higher yields
- Uniform coverage across challenging AT- and GC-rich regions
- Robust amplification from input DNA as low as 250 pg
- Cost-effective alternative to KAPA HiFi with improved performance
sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing. The included primer mix allows amplification of DNA libraries flanked by adapters containing the P5 and P7 sequence elements required for Illumina® sequencing platforms. The hot-start, proofreading DNA polymerase used in the sparQ HiFi PCR Mater Mix is specifically engineered to improve library amplification efficiency while reducing PCR-derived artifacts, resulting in higher library yields and better coverage uniformity. This kit supports low DNA input from 250 pg and efficient amplification of AT- and GC rich regions with minimal bias.
Contents
- 2X HiFi PCR Master Mix
- Primer Mix
- HiFi Enhancer
Storage & Handling
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
Refer to the product label for applicable expiration date.
Resources
Product Manuals
Application Notes
Brochures
CofA (PSFs)
SDSs
Publications
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