sparQ DNA Frag & Library Prep Kit

Features

• High quality libraries in 2.5 hours from 1 ng – 1 μg of input DNA
• Tunable and reproducible fragmentation size range
• Simple, convenient 2-step workflow with minimal hands-on time
• Novel chemistry and high-fidelity amplification minimizing bias
• Superior sequence coverage uniformity and low duplication rate

The sparQ DNA Frag & Library Prep Kit is optimized for enzymatic fragmentation of DNA and streamlined construction of high quality libraries for sequencing on Illumina NGS® platforms. The simple, convenient 2-step workflow can be completed in 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 1 ng to 1000 ng. The DNA fragmentation and polishing reactions are combined in a single step to generate 5′-phosphorylated and 3′-dA-tailed fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-Free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.

NGS Automation
Quantabio sparQ Frag & Library Prep Kit can be fully automated.
Currently sparQ Frag & Library Prep Kit is supported by the following Vendor Automation Platform(s)

• PerkinElmer Applied Genomics SCICLONE G3 NGSx
• Tecan DreamPrep® NGS
• Beckman Coulter (in development)

Storage & Handling
Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

sparQ DNA Frag & Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.