qScript XLT 1-Step RT-qPCR ToughMix

Features

• Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
• Flexible – Use fast or standard qPCR cycling conditions
• Broad dynamic range- Reliable data from your precious samples every time
• Multiplexing enabled – Supports highly sensitive detection for up to four targets

qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.

This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.

Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.

Instrument Compatibility

ROX

• Applied Biosystems 5700
• Applied Biosystems 7000
• Applied Biosystems 7300
• Applied Biosystems 7700
• Applied Biosystems 7900
• Applied Biosystems 7900HT
• Applied Biosystems 7900 HT Fast
• Applied Biosystems StepOne™
• Applied Biosystems StepOnePlus™

Low ROX

• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Stratagene Mx3000P®
• Stratagene Mx3005P™
• Stratagene Mx4000™
• Applied Biosystems ViiA 7
• Applied Biosystems QuantStudio™ (all models)
• Douglas Scientific IntelliQube®

No ROX

• Quantabio Q
• BioRad CFX
• Roche LightCycler 480
• QIAGEN Rotor-Gene Q
• Agilent AriaMx
• Azure Cielo™
• Other

Bio-Rad iCycler iQ systems

• BioRad iCycler iQ™
• BioRad MyiQ™
• BioRad iQ™5

Contents
2X concentrated One-Step Master Mix containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
• qScript XLT reverse transcriptase
• RNase inhibitor protein
• AccuStart II Taq DNA Polymerase
• Inert AccuVue™ plate loading dye
• Proprietary enzyme stabilizers and performance-enhancing additives
• Titrated reference dye (if applicable)

Storage & Handling
Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not affect RT-qPCR performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.