Ready-to-use premixed dNTP solutions for PCR and other DNA synthesis reactions
- Sterile, neutral pH solutions of premixed dGTP, dATP, dTTP and dCTP
- Available at two different concentrations of each dNTP (2.5 mM or 25 mM)
| Name | SKU | Size | Availability | Vendor | Price | Order | |
2′-dNTP (All 4 mixed) |
D59104 | 10 μmole 25 mM ea. | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing |
Sterile, neutral pH, premixed solutions of dATP, dCTP, dGTP, and dTTP are available for use in DNA synthesis reactions including PCR. The premixed dNTPs solutions are available at two concentrations, either 2.5 mM or 25 mM of each dNTP. Each solution is sufficient for at least 1,000 50-µL reactions at concentrations used in typical reactions with most DNA polymerases.
Storage Buffer: dNTP solutions are made with sterile deionized water and adjusted to pH 7.0.
Quality Control: All Epicentre dNTP solutions meet spectrophotometric criteria and are function-tested using our DNA polymerases.
Table 1: Molecular Characteristics of dNTPs
| dATP (2´-Deoxyadenosine-5´-Triphosphate) Formula (free acid): C10H16N5O12P3Molecular weight: 491 A250/A260 = 0.80 A280/A260 = 0.15 |
dCTP (2´-Deoxycytidine-5´-Triphosphate) Formula (free acid): C9H16N3O13P3Molecular weight: 467 A250/A260 = 0.84 A280/A260 = 0.98 |
|
| dGTP (2´-Deoxyguanosine-5´-Triphosphate) Formula (free acid): C10H16N5O13P3Molecular weight: 507 A250/A260 = 1.15 A280/A260 = 0.66 |
dTTP (2´-Deoxythymidine-5´-Triphosphate) Formula (free acid): C10H17N2O14P3Formula weight: 482 A250/A260 = 0.65 A280/A260 = 0.71 |
Manuals and User Guides
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