Name: NxGen T4 DNA Ligase
SKU: 2637
Availability: BackOrder

A workhorse, high quality DNA ligase for joining blunt or cohesive ends of dsDNA or dsRNA.

Key features

Reliably join double-stranded nucleic acid chains.

Name	SKU	Size	Availability	Vendor	Price	Order
NxGen™ T4 DNA Ligase (Low Concentration), 2 U/µL, 1,500 U	30241-1	1,500 U @ 2 U/µL	Generally 1-2 weeks from receipt of order	LGC Biosearch Technologies - Lucigen (Epicentre)			Log in for pricing
NxGen™ T4 DNA Ligase (High Concentration), 10 U/µL, 1,500 U	30243-1	1,500 U @ 10 U/µL	Generally 1-2 weeks from receipt of order	LGC Biosearch Technologies - Lucigen (Epicentre)			Log in for pricing
NxGen™ T4 DNA Ligase Kit High Concentration, 40,000 U at 10,000 U/mL	30243-3	40,000 U @ 10,000 U/µL	Generally 1-2 weeks from receipt of order	LGC Biosearch Technologies - Lucigen (Epicentre)			Log in for pricing

Features

T4 DNA Ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

T4 DNA Ligase Version	Sizes	Ligase Concentration	Provided Buffer
Low Concentration Ligase	1,500 U	2 U/µL	10X T4 DNA Ligase Buffer
	7,500 U
High Concentration Rapid Kit	1,500 U	10 U/µL	2X Rapid Ligation Buffer,
	7,500 U		10X T4 DNA Ligase Buffer

10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl 2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.

2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl 2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.

Purity

Figure 1: Purity
The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining. Molecular weight markers are in Lane 1. T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).

Exonuclease and Endonuclease Activity Assay

Figure 2: Exonuclease and Endonuclease Activity Assay
The T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6). Molecular weight markers are in Lanes 1 and 2.

Product Details

The low concentration (LC) NxGen T4 DNA Ligase Kit includes T4 DNA Ligase at 2 U/µL and 10X T4 DNA ligase Buffer. The high concentration(HC) NxGen T4 DNA Ligase Kit includes NxGen T4 DNA Ligase at 10 U/µL and both 2X Rapid Ligation Buffer and 10X T4 DNA ligase Buffer.