MasterPure™ Yeast RNA Purification Kit

Safe, fast purification of high quality RNA from multiple species of yeast

  • Safe: Avoids the use of dangerous hot acid phenol
  • Fast: Quicker protocol that methods using spheroplasting
  • High Quality RNA: RNA quality exceeds that obtained by bead beating protocols and is ready for use in downstream applications such and RT-PCR and microarray analysis
  • Excellent Yields: Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae
  • Complete: All the necesseary reagents are supplied with no need to purchase extra equipment
Name SKU Size Availability Vendor Price Order
MasterPure™ Yeast RNA Purification Kit MPY03100 100 Purif Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2

References

  1. Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215.
  2. Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.
Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labeled, and hybridized to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labeled with Cy5, and cDNA from control cells was labeled with Cy3. Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Fig. 1. was stored at -20° C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA.

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