MasterPure Complete DNA and RNA Purification Kit

Quickly purify high yields of high-molecular-weight genomic DNA, total cellular RNA or Total Nucleic Acid (TNA) with one kit.

Key features

  • Purify Total Nucleic Acid (TNA), DNA or RNA in 30-60 minutes
  • Safe: Does not use hazardous phenol, chloroform or guanidine
  • High Purity: A260/A280 ratios consistently between 1.8 and 2.0
  • High Yields: Improves yields by avoiding the use of columns which often reduce nucleic acid yields
  • Versatile: Purify TNA, genomic DNA, total RNA, FFPE RNA, or both genomic DNA and total RNA from a sample
  • Total RNA Recovery: Purify both large and small (e.g., miRNA) RNA for RNA-Seq or qRT-PCR
  • Proven: Hundreds of citations for purification of DNA and RNA from dozens of sample types for use in many applications
Name SKU Size Availability Vendor Price Order

MasterPure Complete DNA & RNA Purification Kit, 10 Purif

 MC89010 10 Purif Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

MasterPure Complete DNA & RNA Purification Kit, 200 Purif

 MC85200 200 Purif Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Product information
The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.

The kit procedure uses a safe, gentle, and scalable salt-precipitation protocol (Figure. 1) that eliminates the need for hazardous chemicals and yield-reducing columns. Purification reactions can be scaled up or scaled down depending on the amount of nucleic acid needed and the size of the sample. MasterPure Kits for nucleic acid purification from Gram-positive bacteria, yeast (DNA or RNA), and blood samples are also available.

Applications
Purification of genomic DNA or total RNA for many applications, including:

  • Library preparation for next generation sequencing (NGS) of genomic DNA and RNA
  • DNA methylation studies using Illumina® Infinium® HumanMethylation BeadChips
  • Genomic DNA and cDNA cloning
  • qPCR and qRT-PCR
  • Micrarray analyses (CGH, gene expression profiling, etc)
Epicentre-i_masterpure_completekit
Figure 1. Overview of the MasterPure Complete Kit protocol.
Epicentre-g_masterpure_pcrsensitivity
Figure 2. Comparison of PCR sensitivity using templates obtained using the MasterPure Complete DNA and RNA Purification Kit versus a spin-column kit. PCR amplification was performed after extraction from serial dilutions of E. coli cells ranging from 2 × 10 7 (lanes 1) to 200 (lanes 6). Lane M, DNA marker; lane 7, negative control.
Epicentre-g_masterpure_completekit_6gels
Figure 3. DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure Complete Kit. M = kb ladder.
Targets Samples Extracted
Human & mammalian RNA & genomic DNA Plasma
HIV Whole blood
HCV Guthrie cards
RSV Buccal cells
HPV Liver
Yeast Mouse tail
Enterovirus Kidney
B. pertussis Saliva
E. coli Urine
M. tuberculosis Sputum
Streptococcus mutans Tissue-culture cell lines
Soy (tofu) Cervical cells
Maize Paraffin-embedded tissues
Insect tissues
Table 1. Examples of targets and samples analysed using MasterPure DNA and RNA Purification Kits. Using an extremely simple salt-precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.
Sample Sample Size TNA µg DNA µg RNA µg
HeLa/HL60 cells 1 × 10 6 cells 10-30 3-12 7-15
Tissues
Liver 5 mg 33-42 5-10 13-25
Brain 5 mg 9-13 6-9 4-11
Heart 5 mg 6-10 4-7 4-5
Kidney 5 mg 10-17 3-8 14-17
Thymus 5 mg 15-30 6-12 9-18
Blood 200 µl 3-10 3-9
Buffy coat 300 µl 40-55 40-55 3-6
Mouse tail 0.5 cm 25-30 9-11
E. coli 3.5 × 10 6 cells 2.5-2.8 1.3-1.6 1.6-1.8
S. mutans 1.5 ml 0.9
Yeast* 2.2 × 10 6 cells 11-18
( S. cerevisiae) 1.1 × 10 7 cells 70-78
*For extraction of yeast DNA, the MasterPure Yeast DNA Purification Kit is recommended.
Table 2. Example of yields of nucleic acids obtained using MasterPure Complete DNA and RNA Purification Kits.
Epicentre-g_masterpure_constpurif
Figure 4. Consistent purification of DNA, TNA, and RNA. Five different genomic DNA (lanes 1-5), TNA (lanes 6-10), and total cellular RNA (lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure Complete DNA and RNA Purification Kit. Lane M, Kilobase ladder.

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