Geneaid Presto™ PureXP Magnetic Beads for DNA Cleanup

Magnetic Beads DNA/RNA Extraction/Magnetic Beads PCR Cleanup kit

The Geneaid Presto™ PureXP Magnetic Beads utilizes unique paramagnetic bead technology for high-throughput purification of PCR amplicons. This system uses an optimized buffer to selectively bind DNA fragments of 100 bp or larger to paramagnetic beads. The protocol consists of binding, washing, and elution steps, effectively removing primers, nucleotides, salts, and enzymes from the reaction mixture, resulting in a more purified PCR product. The purified products can be used for downstream applications, such as PCR, sequencing, cloning, genotyping and library construction for NGS.

Name SKU Size Availability Vendor Price Order

PrestoTM PureXP Magnetic Beads for DNA Cleanup

 MXP005 5ml In stock Geneaid Log in for pricing

PrestoTM PureXP Magnetic Beads for DNA Cleanup

 MXP060 60ml Generally 1-2 weeks from receipt of order Geneaid Log in for pricing

PrestoTM PureXP Magnetic Beads for DNA Cleanup

 MXP45096 450ml Generally 1-2 weeks from receipt of order Geneaid Log in for pricing

Specifications

  • Application uses: Purification & Clean-up, PCR Purification, DNA Purification, NGS Clean-up, PCR clean-up
  • Sample Material: PCR amplicons, sheared gDNA
  • Standard Volume Ratio: 1.8x volume of sample
  • Recovered fragment Size: >100 bp
  • Easily adapted to automatic workstations, such as Hamilton Microlab® STAR™
  • Size selection: YES
  • Operation time: within 15 minutes (manual)
  • An ideal substitute for Beckman AMPure XP Beads

 

Applications

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection, and library construction for next-generation sequencing (NGS)

Components

Presto™ PureXP Magbead (5 ml, 60 ml, 450 ml)

Test Data – DNA Recovery

Figure 1.The 50 bp DNA ladder was used to simulate PCR amplicons of varying fragment sizes (50-1,500bp, 1000 ng, 20 µL). Purification was performed using Geneaid Presto™ PureXP Magbeads and B brand Magbeads, respectively (in triplicates). The purified products were quantified using a Qubit (Thermo) and analyzed by capillary electrophoresis. The results demonstrated that the Presto™ PureXP Magbeads exhibited a higher recovery rate (84.6%) compared to the B brand (78.6%). Capillary electrophoresis also showed consistent results.
Figure 2.Sheared DNA (green line) was purified separately using Geneaid Presto™ PureXP Magbeads and B brand Magbeads. After adjusting the concentrations of the purified products to the same level, capillary electrophoresis analysis was performed. The results show that the two curves representing Geneaid Presto™ PureXP Magbeads (red line) and Brand B Magbeads (blue line) completely overlap, confirming that the different DNA fragment sizes recovery profile of Geneaid Presto™ PureXP Magbeads is identical to that of B Brand Magbeads.

Figure 3.Sheared DNA was recovered using Geneaid Presto™ PureXP Magbeads with different volume ratios to remove various DNA fragment sizes. After adjusting the concentrations of the purified products to the same level, capillary electrophoresis analysis was performed. The results showed that a Magbead:DNA ratio of 1.8x effectively removed fragments smaller than 100 bp, a ratio of 0.9x removed fragments smaller than 200 bp, 0.7x removed fragments smaller than 250 bp, and 0.6x removed fragments smaller than 300 bp. Geneaid Presto™ PureXP Magbeads demonstrated optimal size selection functionality.

 

Resources

Presto™ PureXP Magnetic Beads for DNA Cleanup Protocol

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