EverBrite™ Hardset Mounting Medium with NucSpot® 640

Biotium’s hardset antifade mounting medium with NucSpot® 640 far-red nuclear counterstain.

Name SKU Size Availability Vendor Price Order

EverBrite™ Hardset Mounting Medium with NucSpot® 640 (trial size)

 23016-T 2mL Generally 1-2 weeks from receipt of order Biotium Log in for pricing

EverBrite™ Hardset Mounting Medium with NucSpot® 640

 23016 10ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Photos

Features

This antifade mounting medium features Biotium’s NucSpot® 640 nuclear stain, for one-step mounting and nuclear counterstaining.

Features

  • Self-hardening antifade medium with refractive index well-matched to glass
  • Featuring the only far-red nuclear counterstains available in mounting media
  • Avoid cross-talk and photoconversion from DAPI
  • Bright and specific nuclear staining with far-red NucSpot® 640

Spectral Properties

  • NucSpot® 640: 649/668 nm (with DNA) (for Cy®5 channel)

EverBrite™ Hardset Mounting Medium cures to form a hard, permanent seal between the slide and coverslip, eliminating the need to seal the coverslip edges. It cures overnight to a refractive index of 1.42, and continues curing over the course of four days to a refractive index of 1.46. It prevents rapid photobleaching of a wide selection of fluorescent dyes. Unlike VECTASHIELD® Mounting Medium, EverBrite™ is compatible with cyanine-based fluorophores and is optimally formulated for use with Biotium’s CF® Dyes.

This mounting medium features Biotium’s novel NucSpot® 640 is a far-red dye nuclear counterstain for the Cy®5 channel. Using far-red nuclear stains can avoid problems of fluorescence cross-talk and UV photoconversion with traditional dyes like DAPI.

Note: NucSpot® 640 also shows dim blue fluorescence with the DAPI filter set, and should be tested for suitability before using with blue probes, especially for epifluorescence microscopy.

Resources

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