dUTP CF® Dye Conjugates

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

• Synthesize exceptionally bright and photostable fluorescent DNA probes
• Detect by fluorescence microscopy, flow cytometry, or in gel
• Compatible with fixed cells or tissue sections
• Choice of 8 CF® dye colors from blue to near infrared
• Biotium’s CF® dyes have superior brightness, photostability, and are water-soluble

Supplied in lyophilized form. For PCR applications, Taq polymerase should be used with dUTP conjugates, because dUTP inhibits archaeal polymerases such as Pfu and Vent®. Also see our CF® Dye dCTP Conjugates and TUNEL assay kits, available with a wide selection of bright and photostable CF® dyes.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

dUTP CF® Dye Conjugates

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific. Vent is a registered trademark of New England Biolabs, Inc.

REFERENCES

Download a list of curated CF® Dye references.