Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

This is a highly cross-adsorbed donkey anti-mouse IgG (H L) secondary antibody that has a low degree of labelling (DOL) with one of our CF® Dyes validated for STORM.

• CF® Dye single label secondary antibody ideal for STORM imaging
• Highly cross-adsorbed for specific staining with minimal background
• Available in 10 CF® Dyes validated for STORM

Secondary antibodies with a low DOL, or number of dye molecules per antibody molecule, have been reported to be optimal for STORM (Bittel et al. (2015) Proc. SPIE 9331). This product is prepared by single labelling (DOL=1) of highly cross-adsorbed goat anti-mouse IgG (H L) with a selection of compatible CF® Dyes for (d)-STORM super-resolution microscopy. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, horse, human, rabbit, rat, sheep, and Syrian hamster.

Learn more about CF® Dyes for super-resolution microscopy.

View Biotium’s full selection of bright and specific Secondary Antibodies, or search the catalogue using the Antibody Finder. Alternatively, you can view Biotium’s secondary antibody product listings with catalogue numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see Biotium’s CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.