DNA Extraction/Genomic DNA Purification
The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.
| Name | SKU | Size | Availability | Vendor | Price | Order | |
Geneaid Presto™ Bacteria Genomic gDNA Extraction Kit |
GBB100 | 100 Preparation Kit | In stock | Geneaid | Log in for pricing | ||
Geneaid Presto™ Bacteria Genomic gDNA Extraction Kit |
GBB101 | 100 Preparation Kit | Generally 1-2 weeks from receipt of order | Geneaid | Log in for pricing | ||
Geneaid Presto™ Bacteria Genomic gDNA Extraction Kit |
GBB300 | 300 Preparation Kit | Generally 1-2 weeks from receipt of order | Geneaid | Log in for pricing | ||
Geneaid Presto™ Bacteria Genomic gDNA Extraction Kit |
GBB301 | 300 Preparation Kit | Generally 1-2 weeks from receipt of order | Geneaid | Log in for pricing |
Specifications
- Purify genomic DNA within 30 minutes!
- High Yield: up to 40 μg (1 x 109 Escherichia coli) and 15 µg (1 x 109 Bacillus subtilis)
- High Purity gDNA: A260/A280 >1.9
- DNA Size: 20-40 kb
- Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109), 200 µl of blood and biological fluids such as plasma and urine
- Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
- Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
- Elution volume: 30-200 µl
- Storage: dry at room temperature (15-25°C)
Applications
- PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
Components
- Proteinase K
- Gram+ Buffer
- GT Buffer
- GB Buffer
- W1 Buffer
- Wash Buffer
- Lysozyme
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- GD Columns
- 2 ml Collection Tubes
Quality Control
- The quality of the Presto™ Mini gDNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified genomic DNA from Escherichia coli cells (up to 1 x 109), (up to 40 µg with an A260/A280 ratio of >2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Genomic DNA Extraction Kits Specifications
You may also like
-
Tissue & Cell Lysis Solution
Use in combination with Proteinase K to lyse cells and tissues in various samples such as saliva, semen, mammalian cells, and Gram-negative bacteria.
Log in for pricing -
10X QuickExtract Plant DNA Extraction Solution
The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes.
Log in for pricing -
TE Buffer
Standard buffer for storing nucleic acids (10 mM Tris-HCl [pH 7.5], 1 mM EDTA). To be used with the MasterPure Kits and any other nucleic acid extraction/purification kits.
Log in for pricing -
MasterPure™ Yeast RNA Purification Kit
Safe, fast purification of high quality RNA from multiple species of yeast.
Log in for pricing