AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards

A fluorescent dsDNA quantitation kit with a wide linear range of 0.03-250 ng DNA.

Designed for use in a fluorescence microplate reader.

Name SKU Size Availability Vendor Price Order

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size

 31028-T 200 assays Generally 1-2 weeks from receipt of order Biotium Log in for pricing

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards

 31028 1000 assays Generally 1-2 weeks from receipt of order Biotium Log in for pricing

AccuClear® Ultra High Sensitivity dsDNA Quantitation Assay kit with 1 DNA Standard (2000 assays)

 31029 2000 assays Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Features

The AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified DNA samples, designed for use with fluorescence microplate readers. AccuClear® is our most popular DNA quantitation kit, with a very wide linear detection range from 0.03 – 250 ng. The AccuClear® assay is also available without any DNA standards (31027), for those that wish to use their own standard.

Features

Kit Components

  • AccuClear® Dye, 100X
  • DNA Quantitation Buffer, 20X Concentrate*
  • dsDNA Standard, 25 ng/uL (in 2000 assay & trial size kits)
  • dsDNA Standards, Set of 7 (in 1000 assay kit only)
    *Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

 

 

References
1. J Food Sci (2015) May;80(5):M1031-8 doi: 10.1111/1750-3841.12848
2. Analytical Biochemistry (2016) 15 Oct pp74-79 doi:10.1016/j.ab.2016.07.022
3. Microbiome (2018), Oct 20;6(1):188 doi:10.1186/s40168-018-0529-x
4. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102

Resources

Protocol:

SDS:

Supporting Documents:

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