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BIOTIUM | Biotium’s ExoBrite™ Flow Antibody helps validate an upscaling protocol for potential exosome-based therapies
ExoBrite™ CD9 Flow Antibody ORDER HERE AccuOrange™ Protein Quantitation Kit ORDER HERE ExoBrite™ CD9/CD63/CD81 Single-Color Antibody Cocktails ORDER HERE
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BIOTIUM | Protocol: Cell Surface Antibody Staining for Flow Cytometry
There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for different cell types, targets, or applications. This is our basic protocol for extracellular staining of cell surface epitopes in suspension cells for flow cytometry.
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BIOTIUM | Protocol: Immunofluorescence Staining of Cells for Microscopy
Paraformaldehyde, 4% in PBS, Ready-to-Use Fixative ORDER HERE Fish Gelatin Powder ORDER HERE CoverGrip™ Coverslip Sealant ORDER HERE
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BIOTIUM | Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Phalloidin Conjugates ORDER HERE Mix-n-Stain™ CF® Dye Antibody Labelling Kits ORDER HERE
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BIOTIUM | Tech Tip: Battling Tissue Autofluorescence
TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF ORDER HERE TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO ORDER HERE
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Biotium | DNase pre-treatment of the EV exterior improves EV RNA yield
Extracellular vesicles (EVs) are known to contain RNA, including microRNA (miRNA) and mRNA, derived from the cell of origin. Studying EV RNA can be useful for many purposes: determining the cellular source of the EVs, for disease diagnostics (Ref. 1), and for identifying oncogenic RNAs (Ref. 2). Since EVs are very small (~30-200 nm in […]
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Biotium | ExoBrite™ CTB EV Stain assists in evaluating engineered EVs for targeted drug delivery against triple-negative breast cancer
Triple-negative breast cancer (TNBC) is an aggressive cancer type with no specific therapeutic targets, leaving chemotherapy as the primary treatment option. However, chemotherapy is often highly toxic for patients with limited effectiveness, underscoring the need for new therapeutic strategies. Researchers are examining the potential of extracellular vesicles (EVs)- small, cell-derived particles that are naturally highly […]
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Biotium | MitoView™ 633 offers advantages over TMRM as a probe for mitochondrial membrane potential
Mitochondria are essential for maintaining cellular health, with a key aspect of mitochondrial function being the mitochondrial membrane potential (mitochondrial potential). Mitochondrial potential is a valuable biomarker for detecting any disruptions linked to mitochondrial dysfunction. This is because it reflects the electrical gradient central to processes like ATP synthesis and calcium homeostasis across the inner […]
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Biotium | Bright and photostable CF® Dyes help validate fluorescence lifetime separation in multi-colour imaging
Fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerful technique for multi-colour imaging, allowing researchers to distinguish fluorochromes beyond traditional spectral separation methods. By leveraging fluorescence lifetime differences, FLIM provides additional fluorochrome discrimination, enabling more complex imaging experiments with fewer spectral constraints. This approach is particularly valuable in confocal and Stimulated Emission Depletion (STED) […]
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Biotium | New MiniMab™ Single-Domain Antibody Series Provides Super-Resolution Imaging Options for Neuroscience Research
Biotium’s New SYT1, VGLUT1, and GFAP Recombinant Alpaca SdAb conjugates offer exceptional performance for STORM imaging with CF® Dyes Fremont, CA — July 7, 2025 — Biotium, a leading innovator in life science reagents, is proud to support the advancement of super-resolution imaging with the release of their MiniMab™ single-domain antibody (SdAb) series. The MiniMab™ […]
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