Latest News from Biotium

BIOTIUM | Biotium’s cytoplasm and lipid imaging probes

Biotium’s cytoplasm and lipid imaging probes aid discovery of microglial triglyceride metabolism role in neurodegenerative immunity

BIOTIUM | Troubleshooting Tips for Fluorescence Staining

BIOTIUM | Protocol: Fluorescent Western Blotting

To learn more about western blotting, including the advantages of near-infrared fluorescence detection, see Biotium’s webinar: Fundamentals of Western Immunoblotting: Chemiluminescence and NIR Multiplex Imaging (49:44). Materials required: Workflow overview: Total protein prestain kit (optional) Ponceau S (optional) Blocking buffer (see general considerations below) PBS or TBS with 0.1% Tween®-20 (see general consideration below) Primary […]

BIOTIUM | Protocol: DNA Probe Labelling by PCR

This protocol can be adapted to use dUTP or dCTP labelled with other dyes, biotin, or haptens like digoxigenin. See Biotium’s full selection of labelled nucleotides.

BIOTIUM | Protocol: Using Pluronic® F-127 to Solubilize Dyes for Cell Loading

Pluronic® F-127 is a nonionic detergent useful for solubilizing hydrophobic molecules in aqueous solutions. Pluronic® F-127 is commonly used to solubilize hydrophobic AM ester forms of fluorescent indicator dyes for calcium and other ions for loading of the dyes into cells. It also can be used to solubilize other hydrophobic dyes or compounds to facilitate […]

BIOTIUM | Protocol: Succinimidyl Ester Labelling of Protein Amines

Dyes functionalized with succinimidyl ester (NHS Ester or SE) groups can be used to covalently label proteins on primary amines (typically lysine residues) to form a stable amide linkage.

BIOTIUM | Protocol: Maleimide Labelling of Protein Thiol

Maleimide labelling of antibodies can be used as an alternative to succinimidyl ester labelling of amines, for antibodies where amine labelling affects the antibody binding affinity.

BIOTIUM | Protocol: Aminooxy Labelling of Glycoproteins

Aminooxy labelling can be used to conjugate glycoproteins after oxidation of the carbohydrates to generate aldhehyde groups.

BIOTIUM | ExoBrite™ Flow Antibody Conjugates

Validated Antibodies for Detection of EV Markers by Flow Cytometry The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. While antibodies targeting these proteins are available by commercial suppliers, few are validated or perform well for detection of EVs or exosomes. The antibodies and dye labels […]

BIOTIUM | SUPER-RESOLUTION IMAGING

High-Performance EV Stains for Super-Resolution Featuring STORM-Optimized CF® Dyes Characterizing exosomes and EVs by imaging is challenging due to their small size and the resolution limits of conventional light microscopy. Super-resolution techniques like direct stochastic optical reconstruction microscopy (dSTORM) overcome these limits, providing single-molecule resolution of subcellular structures such as EVs. This enables deeper insights […]