News

BIOTIUM | Protocol: Succinimidyl Ester Labelling of Protein Amines

Dyes functionalized with succinimidyl ester (NHS Ester or SE) groups can be used to covalently label proteins on primary amines (typically lysine residues) to form a stable amide linkage.

BIOTIUM | Protocol: Maleimide Labelling of Protein Thiol

Maleimide labelling of antibodies can be used as an alternative to succinimidyl ester labelling of amines, for antibodies where amine labelling affects the antibody binding affinity.

BIOTIUM | Protocol: Aminooxy Labelling of Glycoproteins

Aminooxy labelling can be used to conjugate glycoproteins after oxidation of the carbohydrates to generate aldhehyde groups.

DeNovix | How RNA-Seq Contributes to Single-Cell Sequencing

DeNovix | What’s the Difference Between ATAC-seq and RNA-seq?

Modern genomic research has been tremendously advanced by a myriad of sequencing technologies. Key among these are ATAC-seq and RNA-seq, specialized techniques addressing specific facets of genomic investigation. This article delves into the nuances of these two methods, elucidating their distinctions and applications in nucleic acid sequencing.

LUCIGEN | Nucleic Acid Therapeutics Toolbox: Givosiran, the first liver-targeted siRNA drug

The success of small interfering RNAs (siRNAs) therapeutics in treating rare genetic liver disorders has spurred significant investment to expand these drugs to more common conditions, from hypertension to hepatitis B.1 The growth in the field has only been possible thanks to alterations to the oligonucleotides that enhance siRNA stability and efficacy. These include nucleotide […]

BIOTIUM | ExoBrite™ Flow Antibody Conjugates

Validated Antibodies for Detection of EV Markers by Flow Cytometry The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. While antibodies targeting these proteins are available by commercial suppliers, few are validated or perform well for detection of EVs or exosomes. The antibodies and dye labels […]

BIOTIUM | SUPER-RESOLUTION IMAGING

High-Performance EV Stains for Super-Resolution Featuring STORM-Optimized CF® Dyes Characterizing exosomes and EVs by imaging is challenging due to their small size and the resolution limits of conventional light microscopy. Super-resolution techniques like direct stochastic optical reconstruction microscopy (dSTORM) overcome these limits, providing single-molecule resolution of subcellular structures such as EVs. This enables deeper insights […]

BIOTIUM | ExoBrite™ Antibody Cocktails

Streamline Your Workflows with High-Coverage EV Staining or Robust Phenotype Analysis Biotium’s ExoBrite™ CD9/CD63/CD81 Antibody Cocktails are antibody mixtures developed with optimal brightness and signal-to-noise for detecting EVs. The single-colour cocktails were developed for high-coverage EV antibody staining by flow cytometry. For phenotyping analysis, the 3-colour cocktails provide distinct fluorescent labels for each marker, enabling […]

Co-Dx | The History of Tuberculosis Diagnostics

Tuberculosis has a complicated history as an endemic killer for thousands of years, with infection rates continuing to rise regardless of advances in modern medicine. The first step to stemming the tide is accurate diagnoses, leading to prompt treatment. Experts have identified several elements to TB testing that need to evolve before health care systems […]