LGC BIOSEARCH Webinars

 

Leveraging the Power of In Vitro Transcription

Abstract

RNA was long underrated as simply the cellular messenger of genetic information between DNA and expressed proteins. It’s now clear that RNA molecules play many roles within cells in addition to translation (mRNA), such as translation inhibition (miRNA), bacterial defense (CRISPR gRNA) and as transmission of genetic material for many viruses.

In vitro transcribed RNA is an integral component in many research projects aimed at understanding these important cellular functions and viruses. The speed and scale of RNA production, as well as the ability to incorporate chemically modified nucleotides, support an array of exciting research applications.

Key Learning Objectives

• Review in vitro transcription (IVT) technology
• Summarize applications of IVT RNA including examples from the literature
• Discuss some of the key challenges of IVT
• Provide potential solutions to those challenges including the AmpliScribe™ T7-Flash and DuraScribe® T7 Transcription Kits

Speaker

Rob Brazas, Ph.D.
Senior Product Manager, Lucigen