TyraMax™ Amplification Dyes and Kits

TyraMax™ Dyes are Biotium’s next generation of tyramide amplification dyes for spatial biology. The dyes have been designed to yield a brighter signal compared to our original CF® Dye tyramides, and have advantages in brightness, photostability, and working solution stability compared to other TSA dyes.

Features

• Largest selection of tyramide colors available, with unique options from blue to near-IR
• Stable in amplification buffer for at least 24 hours, including near-IR dyes
• Advantages in brightness and/or photostability compared to other TSA dyes
• Available as standalone dyes with trial sizes, and dye sets for multiplexing or panel development

Power Your Multiplex Panels with TyraMax™

Tyramide signal amplification (TSA), also known as Catalyzed Reporter Deposition (CARD), is a highly sensitive technique for detecting low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (FISH). The superior sensitivity of TSA and its compatibility with multiplexing by cyclic immunofluorescence (CycIF) has made it indispensable for spatial biology applications.

TyraMax™ Dyes were developed as high-performance TSA dyes, offering brighter, more photostable signals than Aluora® and Opal® reagents. They also remain stable in amplification buffer for up to 24 hours, facilitating automated staining workflows. For optimal results, Biotium recommends using Tyramide Amplification Buffer Plus (Cat. No. 22029) with TyraMax™ Amplification Dyes.

Widest Selection of Colors for Unrivaeled Panel Flexibility

PFA-fixed HeLa cells stained with WGA-HRP detected with TyraMax™ Amplification Dyes in Biotium’s Tyramide Amplification Buffer Plus. Imaged using a 40X oil objective on Evident FV4000 confocal system.

Superior Brightness Compared to Other TSA Reagents

TyraMax™ 430/500 has excellent brightness compared to other commerically available TSA dyes. PFA-fixed HeLa cells stained with WGA-HRP and detected with TyraMax™ 430/500 Amplification Dye, Opal® Polaris 480 Reagent (Ex/Em 450/500 nm), or Aluora® 430 Spatial dye in Biotium’s Tyramide Amplification Buffer Plus. Imaged using a 40X oil objective on Evident FV4000 confocal system with 405 nm excitation in the Alexa Fluor® 430 detection channel.

Signal Stability You Can Count On

TyraMax™ 630/650 has excellent brightness and photostability compared to other commercially available TSA dyes. PFA-fixed HeLa cells stained with WGA-HRP and detected with TyraMax™630/650 Amplification Dye, Opal™ Polaris 650 Reagent, or Aluora™ 647 Spatial dye. Imaged using a 40X oil objective on Evident FV4000 confocal system with 640 nm excitation in the Alexa Fluor® 647 detection channel. Photostability measurements were done on an Olympus IX71 epifluorescence microscope using a Cy®5 filter cube. Stained cells were imaged every 10 seconds for 120 seconds, and mean fluorescence was normalized to the first image taken.

TyraMax™ 740/770 is stable in working solution for at least 24 hours. OD-matched solutions of TyraMax™ 740/770 and Aluora® 750 dyes were prepared in Tyramide Amplification Buffer Plus with 0.0015% hydrogen peroxide. Absorbance was measured on an Agilent Cary 60 spectrophotometer, and then the solutions were left at room temperature, protected from light, and absorbance was measured at various time points up to 24 hours. TyraMax™ 740/770 was stable in the presence of hydrogen peroxide over 24 hours, while Aluora® 750 completely degrades overnight.

TyraMax™ Amplification Dyes are available with a wide choice of dye options for multiplexing by conventional or spectral imaging. TyraMax™ Dyes are offered as standalone dye solutions, as 3-color or 5-color dye sets plus DAPI counterstain, and in a sampler for custom panel optimization. See the tables below for a full list of TyraMax™ Dyes, kits, and recommended channels.

TyraMax™ Amplification Dyes

TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)

TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)

TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)

Aluora, and Alexa Fluor are registered trademarks of Thermo Fisher Scientific; Opal is a registered trademark of Akoya Biosciences; CY DYE is a registered trademark of Cytiva.