TG1 Electrocompetent Cells

TG1 Electrocompetent Cells, 12 Reactions (DUOs)

Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.

Key features

  • Perfect for antibody phage display library creation
  • The highest efficiency TG1 competent cells available.
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells
Name SKU Size Availability Vendor Price Order

TG1 Electrocompetent Cells, 12 Reactions (DUOs)

 60502-1 12 reactions Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

TG1 Electrocompetent Cells, 24 Reactions (DUOs)

 60502-2 24 reactions Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Features

Product Details

Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Product information

TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression

Transformation efficiency

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor’s specification.

Table 1. Competent Cells for Phage Display libraries

Cell Lines Transformation Efficiency
(cfu/µg pUC DNA)		 Cloning Methylated DNA BAC, Cosmid Cloning Blue/White Screening
TG1 Electrocompetent ≥4 × 10¹⁰ YES NO YES IPTG induction required
SS320 (MC1061 F’) Electrocompetent ≥4 × 10¹⁰ YES NO YES IPTG induction required
MC1061 F-Electrocompetent ≥4 × 10¹⁰ YES NO YES IPTG induction required
ER2738 Electrocompetent ≥2 × 10¹⁰ YES NO YES IPTG induction required

Product specifications and usage

Genotype

  • TG1: [F’ traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK – mK -)
  • SS320 (MC1061F’): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
  • ER2738: [F’proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
  • MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1

Resources

Product information sheets

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