SynaptoRed™ C2 (Equivalent to FM®4-64)

SynaptoRed™ C2

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.

Name SKU Size Availability Vendor Price Order
SynaptoRed™ C2 (Equivalent to FM®4-64) Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Product Description

A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia. Please also see AM4-64 (70025), which is similar to SynaptoRed™, but with a formaldehyde-fixable amine group.

SynaptoRed™ can also be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λEx/λEm (membranes) = ~510/750 nm*
  • Dark purple solid soluble in water
  • C30H45Br2N3
  • MW: 608
  • [162112-35-8]

SynaptoRed C2 (FM®4-64)

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed C2 (FM®4-64) (Ref. 4).

Nerve Terminal Dyes and Kits

m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.

FM is a registered trademark of Thermo Fisher Scientific.

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