Steady-Luc™ Firefly HTS Assay Kit (Lyophilized)

Features

The Steady-Luc™ HTS assay system is a homogeneous, high-sensitivity firefly luciferase reporter gene assay kit. It is a glow-type assay with signal half-life of about 3 hours, allowing batch processing in microplates.

Features

• Simple, single step homogeneous assay
• Luminescence half life of about 3 hours
• Lyophilized assay buffer for convenient shipping & storage
• Great value: save up to 45% compared to other brands

Kit Components

• D-Luciferin
• Steady-Luc™ Firefly Assay Buffer (Lyophilized)
• Reconstitution Buffer

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.

Biotium’s Steady-Luc™ Firefly Assay Buffer contains a proprietary mixture of substances that prevent the adenyl-oxyluciferin reaction, producing a long lasting, glow-type signal. It produces luminescence signal with a half-life of about 3 hours, for a high-throughput microplate assay.

Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.

This product is comparable to our original Steady-Luc™ HTS Assay (catalog no. 30028), but with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. Simply dissolve the lyophilized assay buffer in the supplied reconstitution buffer before use.