Steady-Luc™ Firefly HTS Assay Kit

Features

The Steady-Luc™ HTS assay system is a homogeneous, high-sensitivity firefly luciferase reporter gene assay kit. It is a glow-type assay with signal half-life of about 3 hours, allowing batch processing in microplates.

Features

• Simple, single step homogeneous assay
• Luminescence half life of about 3 hours
• Great value: save up to 45% compared to other brands

Kit Components

• D-Luciferin
• Steady-Luc™ Firefly Assay Buffer

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.

Biotium’s Steady-Luc™ Firefly Assay Buffer contains a proprietary mixture of substances that prevent the adenyl-oxyluciferin reaction, producing a long lasting, glow-type signal. It produces luminescence signal with a half-life of about 3 hours, for a high-throughput microplate assay.

Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.

Biotium also offers Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) (catalog no. 30028L), a new packaging format that includes lyophilized assay buffer for convenient room temperature shipping and storage at -20°C.