SS320 (MC1061 F’) Electrocompetent Cells, 12 Reactions (DUOs)
Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
Key features
- Perfect for antibody phage display library creation
- Create larger libraries; speed discovery
- Bulk custom dispensing available with all of our phage display cells
| Name | SKU | Size | Availability | Vendor | Price | Order | |
SS320 (MC1061 F’) Electrocompetent Cells, 12 Reactions (DUOs) |
60512-1 | 12 reactions | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing | ||
SS320 (MC1061 F’) Electrocompetent Cells, 24 Reactions (DUOs) |
60512-2 | 24 reactions | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing |
Features
Product Details
Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
Product information
SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F’) prepared as highly efficient (≥4 × 10¹⁰ cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor’s specification.
Table 1. Competent Cells for Phage Display libraries
| Cell Lines | Transformation Efficiency (cfu/µg pUC DNA) |
Cloning Methylated DNA | BAC, Cosmid Cloning | Blue/White Screening |
|---|---|---|---|---|
| TG1 Electrocompetent | ≥4 × 10¹⁰ | YES | NO | YES IPTG induction required |
| SS320 (MC1061 F’) Electrocompetent | ≥4 × 10¹⁰ | YES | NO | YES IPTG induction required |
| MC1061 F-Electrocompetent | ≥4 × 10¹⁰ | YES | NO | YES IPTG induction required |
| ER2738 Electrocompetent | ≥2 × 10¹⁰ | YES | NO | YES IPTG induction required |
Product specifications and usage
Genotype
- TG1: [F’ traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK – mK -)
- SS320 (MC1061F’): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
- ER2738: [F’proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
- MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
Resources
SDS
Manuals and User Guides
Product information sheets
Application notes
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