sparQ mRNA-Seq Kit

Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture

Features & Benefits

  • Highly sensitive mRNA detection with input as low as 5 ng of total RNA
  • Reproducible transcript profiling
  • High quality directional RNA library prep with streamlined workflow of 4.75 hours
  • Efficient isolation of mRNA allowing detection of most coding genes

sparQ mRNA-Seq Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Name SKU Size Availability Vendor Price Order

sparQ mRNA-Seq Kit, 8R

 95218-008 8 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

sparQ mRNA-Seq Kit, 24R

 95218-024 24 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

sparQ mRNA-Seq Kit, 96R

 95218-096 96 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Description
sparQ mRNA-Seq Kit enables efficient preparation of stranded mRNA-seq libraries for Illumina® NGS platforms. This workflow reduces hands-on time and delivers reliable mRNA-seq libraries from as little as 5 ng and up to 1 μg of total RNA. The streamlined workflow generates high quality, directional mRNA libraries that are consistent and with reduced bias.

sparQ UDI Adapter Resources
Setting up sparQ Library Prep kits in Illumina Experiment Manager


Contents

Box contents
*The ‘Binding Buffer’ is in a clear-capped bottle for the 96 reaction kit. The ‘Wash Buffer’ is in a clear-capped bottle for the 24 and 96 reaction kits. Otherwise, they are in a tube with a yellow cap.

Storage & Handling
Store Box 1 in a constant temperature refrigerator at 2°C to 8°C upon receipt.
Store Box 2 in a constant temperature freezer at -25°C to -15°C upon receipt.

  • For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Performance Data

sparQ mRNA-Seq Kit workflow delivers a simple workflow with a rapid protocol that allows for sample to sequencing in a single day
sparQ-mRNA-Seq-Kit-Streamlined_Workflow
sparQ mRNA-Seq Kit demonstrates reproducibility across biological replicates
sparQ-mRNA-Seq-Kit-Fig2-Reproducible-Transcript-Profiling
sparQ mRNA-Seq Kit enables preparation of libraries with low input amounts, down to 5 ng
sparQ-mRNA-Seq-Kit-High-Sensitivity
sparQ mRNA-Seq Kit demonstrates excellent performance on all Illumina sequencing platforms
Excellent-Sequencing-Metrics

Resources

Product Manuals

Brochures

CofA (PSFs)

SDSs

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