sparQ DNA Library Prep Kit

Streamlined, versatile single-tube solution for high quality library prep
Name | SKU | Size | Availability | Vendor | Price | Order | |
sparQ DNA Library Prep Kit, 24 Rxns |
95191-024 | 24 Rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing | ||
sparQ DNA Library Prep Kit, 96 Rxns |
95191-096 | 96 rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing |
Features
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- Fast, easy single-tube solution completes library prep in 2.5 hours
- Suitable for a wide range of input amounts from as low as 250 pg
- Optimized chemistry ensuring superior library prep sensitivity and efficiency
- Higher library yields compared to other library prep kits
- High efficiency enables PCR-free workflow from 100 ng input
sparQ DNA Library Prep Kit is optimized for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms. The simplified protocol speeds up library prep to 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 250 pg to 1 ug. DNA polishing reactions are streamlined into a single step to convert fragmented DNA into 5′-phosphorylated and 3′-dA-tailed DNA fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.
NGS Automation
Quantabio sparQ Frag & Library Prep Kit can be fully automated.
Currently sparQ Frag & Library Prep Kit is supported by the following Vendor Automation Platform(s)
- PerkinElmer Applied Genomics SCICLONE G3 NGSx
- Tecan DreamPrep NGS
- Beckman Coulter (in development)
sparQ DNA Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Resources
Flyers
Product Manuals
sparQ UDI Adapter Resources
Setting up sparQ Library Prep kits in Illumina Experiment Manager
Technical Note – Setting up sparQ Library Prep Kit in Illumina Experiment Manager
Illumina Experiment Manager version 1.8 files (zip-download)
Illumina Experiment Manager version 1.9 files (zip-download)
sparQ UDI Adapter sequence overview
Application Notes
- Rapid 16S Metagenomic Library Preparation for Oxford Nanopore Technologies (ONT)® Platform
- sparQ UDI Adapter sequence overview
- Illumina Experiment Manager version 1.9 files (zip-download)
- Illumina Experiment Manager version 1.8 files (zip-download)
- MK-TN-0009_REV_01_IEM_Set-up_Guide_Using_sparQ_Adapter_Barcode.pdf
- Simple, high quality, and cost-effective library preparation solutions for NGS
- Setting up sparQ Library Prep Kit in Illumina Experiment Manager
- Automation of the sparQ DNA Library Prep Kit on the PerkinElmer® Sciclone G3
Brochures
CofA (PSFs)
- PSF-95191-096-LOT-66222955.pdf
- PSF-95191-024-LOT-66222545.pdf
- PSF-95191-096-LOT-66215580.pdf
- PSF-95191-024-LOT-66215573.pdf
- PSF-95191-096-LOT-66214603.pdf
SDSs
Publications
A comprehensive study of a 29-capsid AAV library in a non-human primate central nervous system
Oleksandr Kondratov – 2021
Transient upregulation of IRF1 during exit from naive pluripotency confers viral protection
Merrit Romeike – 2022
Six de novo assemblies from pathogenic and non-pathogenic strains of Fusarium oxysporum f. sp. niveum
James C. Fulton – 2021
Six de novo assemblies from pathogenic and non-pathogenic strains of Fusarium oxysporum f. sp. niveum
James C. Fulton – 2021
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