sbeadex Lightning Cell DNA Kit

Features

Lucigen’s new sbeadex™ Lightning Cell DNA Kit enables high-speed purification using an innovative binding principle that allows simultaneous binding and washing of DNA.

The patent-pending magnetic bead technology purifies high-quality DNA from human and animal cells in a simple 4-step process, typically requiring only 11 minutes* from sample to eluate.

With reduced hands-on steps, removing a washing and a drying step, sbeadex Lightning is easy for both manual and automated workflows and is compatible with most popular robotic platforms for high-throughput. Fewer protocol steps also significantly reduces liquid and plastic waste for improved sustainability.

* refers to incubation times only and includes cell lysis

Key Features:

• High DNA quality and yield: Attain highly purified DNA in high yields suitable for a wide range of downstream applications including NGS and qPCR
• Save time and increase sample throughput: Superfast and convenient protocols that is easy to scale up and automate on popular open access platforms including KingFisher, Dynamic Devices, Hamilton and our oKtopure.
• Cost savings: Benefit from a 4-step protocol that reduces overall lab costs (e.g. plastic consumables, waste disposal) and ensures reliable and reproducible results
• Sustainability and user safety: reduced plastic consumption, no chaotropic or ethanol bases wash buffers – reduced hazard and reduced packaging waste vs. traditional mag bead based kits.

Why switch to Lightning?

• Superior DNA yields and purity in typically 11 minutes*
• Simplified workflow – only 4 protocol steps
• No ethanolic or high-salt wash buffers
• Suitable for automation and high-throughput workflows
• Save plastic consumables (≤70%), waste disposal (50-80%) and shipping costs

Superior DNA quality in a fraction of the time from your human and animal cells
Summary of the key savings for the sbeadex Lightning Cell DNA Kit compared to market-leading competitors.

This schematic illustrates the savings in protocol time, protocol steps, plastic consumables and liquid waste per sample. Values are based on processing HEK293 cells. Protocol time per sample (minutes) refers to incubation times only and includes cell lysis. Number of protocol steps includes lysis. Liquid waste (mL) excludes elution volume.

DNA extraction and purification from human and animal cells
The sbeadex Lightning Cell DNA Kit is effective for a variety of cell types, including HeLa, HEK293 and CHO cell cultures and peripheral blood mononuclear cells (PBMCs). The kit is suitable for DNA isolation from ≤1×106 cells per reaction.

Ideal for workflows that require screening large numbers of cell culture samples, such as quality control of gene editing events.

How does sbeadex Lightning work?
sbeadex magnetic beads enable automated high-throughput extraction and purification of high-quality nucleic acids from a variety of cell types.

The superparamagnetic particles, coated with sbeadex surface chemistry, employ a novel binding mechanism that ensures effective removal of impurities from the sample matrix. This innovative technology cuts time to only 11 minutes by requiring only a binding buffer, our proprietary sbeadex particles, and an elution buffer. The wash step is performed with water, preventing organic/alcoholic traces in the eluate that might inhibit enzymatic downstream applications. This eliminates the need for a drying step before elution, further enhancing DNA quality.

The fast and simple 4-step protocol delivers high-quality, high-yield DNA that easily draws level with other popular purification kits.

Sustainability
sbeadex Lightning significantly reduces liquid and hazardous waste as well as plastic consumption and energy for shipping, storage and instrumentation — reducing CO2 emissions and cost too.
This fast, simple and convenient workflow is more environmentally friendly than other comparable kits in the market.

Product Details:

The sbeadex Lightning Cell DNA Kit contains all reagents to isolate high-quality DNA from human and animal cells.

The Kit contains:

• Lysis Buffer CLA
• Protease K solution
• RNase A solution
• sbeadex particle suspension
• Binding Buffer LCL
• Elution Buffer AMP

Choose between 96 or 960 preparations, or enquire for bulk options.