qScript cDNA SuperMix

Superior cDNA synthesis in a Single Step.

Name SKU Size Availability Vendor Price Order

qScript cDNA SuperMix Sample Size
25 x 20 μL rxns (1 x 100 µL)

 95048-025 25 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

qScript cDNA SuperMix
100 x 20 μL rxns (1 x 400 µL)

 95048-100 100 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

qScript cDNA SuperMix
500 x 20 μL rxns (2 x 1000 µL)

 95048-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Photos

Features

qScript cDNA SuperMix is a sensitive and easy-to-use 1-tube reagent for first-strand cDNA synthesis that combines a highly-modified RNAse H+ mutant of M-MLV together with ribonuclease inhibitor protein (RIP) in a rigorously optimized formulation for real-time qPCR applications. The stabilized SuperMix formulation has been rigorously optimized to deliver sensitive, linear assay performance across a spectrum of relative abundance and input RNA (10pg – 1ug). qScript cDNA SuperMix reagent performance is unaffected by repetitive freeze/thaw cycling (>20X), conferring greater ease-of-use and consistent assay performance. Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5′ and 3′-sequences for accurate gene expression quantification. For gene-specific priming (GSP) or two-step RT-PCR of RNA exceeding 1kb total length, see our qScript Flex cDNA Kit.

qScript cDNA SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Contents

  • Single-tube, 5X concentrated reagent containing:
  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • Recombinant ribonuclease inhibitor protein (RIP)
  • qScript reverse transcriptase
  • Titrated concentrations of random hexamer and oligo(dT) primer
  • Proprietary enzyme stabilizers and performance-enhancing additives

Storage & Handling

  • Store components in a constant temperature freezer at -25°C to -15°C upon receipt. Repeated freezing and thawing does not affect functional performance. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
  • Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. 5X concentrated reagent is viscous. Do not immerse pipet tip below liquid surface when drawing out material.

Resources

Flyers

Product Manuals

Application Notes

CofA (PSFs)

SDSs

FAQs

  • Is MMLV RT as stable in the qScript cDNA Supermix as in the qScript cDNA synthesis kit? Do you recommend storing these products at -80C?

    In the qScript cDNA synthesis kit, the RT/RNase inhibitor mix is provided as a concentrated enzyme separately from the 5X reaction mix. This enzyme is actually stable at -20C for a year. The cDNA SuperMix has a shelf life of 12 months at -20C. Neither product shows any diminished performance after storage in a REVCO at -70C to -80C; two years for the qScript cDNA Supermix and five years for the qScript cDNA synthesis kit. If you are looking to prolong shelf life, storage at -80C is best for the qScript cDNA Supermix. Storage at -20C is sufficient for the qScript cDNA Synthesis Kit.

  • I am trying to amplify a very rare transcript. Can I scale up a cDNA synthesis reaction and use 2ug of mRNA?

    qScript cDNA SuperMix can easily handle up to 2 ug of total RNA in a typical 20-uL first-strand reaction without compromising cDNA synthesis efficiency. For the qScript cDNA Synthesis Kit we recommend doubling the reaction volume to 40-uL. Simply scale each component proportionally.

  • Do the cDNA Supermix and the cDNA synthesis Kits have the same dynamic range in RNA quantity?

    Both qScript cDNA Supermix and qScript cDNA Synthesis Kits have a broad linear dynamic range. We recommend using them for first-strand cDNA synthesis using HeLa cell total RNA dilutions ranging from 1 µg to 1 pg. One tenth of each first-strand reaction should be used for qPCR amplification. Within this range of HeLa total RNA, qPCR results should be equivalent with both kits. qScript cDNA synthesis kit may have advantages over the qScript cDNA Supermix in certain situations such as working with small quantities of RNA. qScript cDNA Supermix outperforms the qScript cDNA Synthesis kit when starting with quantities of total RNA in excess of 100 ng. qScript cDNA SuperMix can easily handle up to 2 ug of total RNA in a typical 20-uL first-strand reaction without compromising cDNA synthesis efficiency. With the qScript cDNA Synthesis Kit we recommend doubling the reaction volume to 40-uL and simply scaling each component proportionally.

  • What is the difference between qScript cDNA SuperMix and the qScript cDNA Synthesis kit? 

    qScript cDNA SuperMix is the first “”true”” SuperMix format commercially available for cDNA synthesis. A single tube reaction, of 5X concentrated master mix, provides all necessary components for first-strand synthesis (except RNA template) including: buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, qScript reverse transcriptase and stabilizers. In the qScript cDNA Synthesis kit the qScript RT (mixture of RNase inhibitor protein and qScript reverse transcriptase) is provided as a concentrated enzyme. A separate tube contains a solution of 5X concentrated reaction buffer, dNTPs, MgCl2, and primers. Aside from the inclusion of the enzyme and the stabilizers in the qScript cDNA SuperMix, its formulation is also slightly different from the qScript cDNA Synthesis formulation.

  • What is the RNA input for qScript cDNA Supermix?

    The qScript cDNA Supermix provides for the quantitative conversion of 1µg to 10 pg total RNA to cDNA, with a reaction volume of 20 ul.

Publications

The Role of Key Schistosoma mansoni Invadolysins in Infection Establishment and Persistence in Biomphalaria glabrata and Mammalian Hosts: Functional Characterization of SmLeish and SmCI-1
Jacob Riley Hambrook – 2023

RAB27B controls palmitoylation-dependent NRAS trafficking and signaling in myeloid leukemia
Jian-Gang Ren – 2023

Developmental origins of heterogeneity in melanocytes and implications for melanoma
Ryan M. Samuel – 2023

MYC regulates CSF1 expression via microRNA 17/20a to modulate tumorassociated macrophages in osteosarcoma
Bikesh K. Nirala – 2023

Bile salts and proinflammatory cytokines inhibit MCT1-mediated cellular uptake of butyrate and interfere with its antiproliferative properties
Mafalda R. Couto – 2023

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