This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.
| Name | SKU | Size | Availability | Vendor | Price | Order | |
| PMA Enhancer for Gram Negative Bacteria, 5X Solution | 31038 | 16mL | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing |
Photos
Features
PMA Enhancer for Gram Negative Bacteria improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.
- Used in viability PCR with PMA or PMAxx™
- Decreases dead cell signal in gram-negative bacteria
- Should not be used with gram-positive bacteria
- Provided as a 5X solution
The mechanism by which Enhancer improves live/dead discrimination has not been determined. It may improve passive permeability of dead cell walls or membranes to the dye, and/or improve access of the dye to the dead cell DNA.
Viability PCR
Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal qPCR does not distinguish between live and dead cells. With v-PCR using PMAxx™ or PMA, you get the speed, sensitivity and specificity of PCR, plus quantifiable viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses, eukaryotes, and archaea.
To learn more about the advantages of determining microbial or cell viability using viability PCR, visit Biotium’s Viability PCR Technology Page.
Resources
Cell Viability & Cell Death Brochure:
Download list of curated PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.
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