Phalloidin Conjugates

A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.

Name SKU Size Availability Vendor Price Order

Biotin-XX Phalloidin 100U

 28 100 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®350 Phalloidin 347/448 nm – 50U

 00049-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®350 Phalloidin 347/448 nm – 300U

 49 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®405M Phalloidin 408/452 nm – 50U

 00034-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®405M Phalloidin 408/452 nm – 300U

 34 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®430 Phalloidin 426/498 nm – 50U

 00054-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®430 Phalloidin 426/498 nm – 300U

 54 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®440 Phalloidin 440/515 nm – 50U

 00055-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®440 Phalloidin 440/515 nm – 300U

 55 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®488A Phalloidin 490/515 nm M- 50U

 00042-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®488A Phalloidin 490/515 nm M- 300U

 42 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®532 Phalloidin 527/558 nm – 50U

 00051-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®532 Phalloidin 527/558 nm – 300U

 51 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®543 Phalloidin 541/560 nm – 50U

 00043-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®543 Phalloidin 541/560 nm – 300U

 43 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®568 Phalloidin 562/583 nm – 50U

 00044-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®568 Phalloidin 562/583 nm – 300U

 44 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®583R Phalloidin 586/609 nm – 50U

 00064-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®583R Phalloidin 586/609 nm – 300U

 64 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®594 Phalloidin 593/614 nm – 50U

 00045-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®594 Phalloidin 593/614 nm – 300U

 45 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®633 Phalloidin 630/650 nm – 50U

 00046-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®633 Phalloidin 630/650 nm – 300U

 46 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®640R Phalloidin 642/662 nm – 50U

 00050-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®640R Phalloidin 642/662 nm – 300U

 50 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®647 Phalloidin* 650/665 nm – 50U

 00041-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®647 Phalloidin* 650/665 nm – 300U

 41 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®660C Phalloidin CF®660C – 50U

 00052-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®660C Phalloidin CF®660C – 300U

 52 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®660R Phalloidin 663/682 nm – 50U

 00047-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®660R Phalloidin 663/682 nm – 300U

 47 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®680 Phalloidin* 681/698 nm – 50U

 00053-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®680 Phalloidin* 681/698 nm – 300U

 53 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®680R Phalloidin 680/701 nm – 50U

 00048-T 50 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

CF®680R Phalloidin 680/701 nm – 300U

 48 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Fluorescein Phalloidin 496/516 nm – 300U

 30 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Rhodamine Phalloidin 540/565 nm – 300U

 27 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Rhodamine 110 Phalloidin 502/524 nm – 300U

 32 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Sulforhodamine 101 (Texas Red®) Phalloidin 591/608 nm – 300U

 33 300 U Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Features

A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized cells.

  • Choice of 17 bright & photostable CF® Dyes, biotin, or other common fluorophores
  • Dye options for super-resolution and 2-photon imaging
  • Excellent signal to background with negligible non-specific staining

Fluorescently-labeled phalloidins bind to F-actin with nanomolar affinity. Labeled phalloidins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants, animals and fungi. Different from antibodies, the binding affinity of phalloidin does not change significantly with actin among different species.

Fluorescently-labeled phalloidin is typically used to stain fixed and permeabilized cells. Labeled phalloidins are not cell-permeant and have therefore not been used extensively with living cells, however they can be loaded into live cells via cationic liposomes.

Note: Phalloidin stock solutions are very stable. But binding of fluorescent phalloidins to cells is more labile than other probes, such as antibodies, and staining can become non-specific or lost over time. For best results, store phalloidin-stained samples in a suitable mounting medium at 4°C, protected from light. For certain phalloidin conjugates, especially CF®405M, CF®647, and CF®680, we recommend imaging immediately or shortly after staining. Staining with our other CF® Dye phalloidins usually is stable for up to a week when specimens are stored at 4°C, protected from light.

Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Super-Resolution Microscopy
CF®647 and CF®680 phalloidins are recommended mainly for STORM applications. Certain CF® Dyes are compatible with various super resolution imaging techniques. The superior brightness, photostability, and photochemical switching properties of certain CF® Dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® Dyes for super-resolution microscopy.

*CF®647 and CF®680 phalloidins are recommended for STORM applications, but due to the instability of staining with these conjugates, we do not recommend using them for other microscopy applications. For other applications requiring far-red phalloidins, we recommend CF®633, CF®640R, or CF®680R phalloidins, which have more stable binding.
Note: CF®555 Phalloidin (00040, 00040-T) has been discontinued. We recommend CF®568 Phalloidin as a replacement for conventional microscopy, or CF®583R Phalloidin as a replacement for STORM.

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

Resources

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