PerfeCTa SYBR® Green SuperMix

Sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry

Name SKU Size Availability Vendor Price Order

PerfeCTa SYBR Green SuperMix for iQ
500 x 50 μL rxns (10 x 1.25 mL)

 95053-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix for iQ
2000 x 50 μL rxns (1 x 50 mL)

 95053-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix
500 x 50 μL rxns (10 x 1.25 mL)

 95054-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix
2000 x 50 μL rxns (1 x 50 mL)

 95054-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix ROX
500 x 50 μL rxns (10 x 1.25 mL)

 95055-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix ROX
2000 x 50 μL rxns (1 x 50 mL)

 95055-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix Low ROX
500 x 50 μL rxns (10 x 1.25 mL)

 95056-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa SYBR Green SuperMix Low ROX
2000 x 50 μL rxns (1 x 50 mL)

 95056-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Photos

Features

PerfeCTa SYBR® Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStart™II antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.

Features & Benefits

  • 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
  • Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
  • Supports efficient vortex mixing with proprietary anti-foaming formulation
  • SuperMix version provides maximum dye concentration for robust optical signal with small amplicons (i.e. microRNAtemplated cDNA)

PerfeCTa SYBR Green SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Contents

  • Single-tube, 2X concentrated reagent containing:
  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
  • AccuStart II Taq DNA Polymerase
  • SYBR Green I dye
  • Proprietary enzyme stabilizers and performance-enhancing additives.
  • Titrated reference dye (if applicable).

Storage & Handling

  • Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
  • After thawing, mix thoroughly before using.
  • For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Resources

Flyers

Product Manuals

Application Notes

CofA (PSFs)

SDSs

FAQs

Publications

Molecular networking identifies an AHR-modulating benzothiazole from white button mushrooms (Agaricus bisporus)
Xiaoling Chen – 2023
Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to Ultraviolet Exposure
Hyeongsun Moon – 2017
Week-Old Chicks with High Bacteroides Abundance Have Increased Short-Chain Fatty Acids and Reduced Markers of Gut Inflammation
Yi fan – 2023
Quantifying the effect of grilling and roasting on the eating quality of lamb leg muscles
Al Moadhen H – 2022
Immune sensing of food allergens promotes aversive behaviour
Esther B. Florsheim – 2023

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