PerfeCTa qPCR SuperMix

A 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems

Name SKU Size Availability Vendor Price Order

PerfeCTa qPCR SuperMix
500 x 50 μL rxns (10 x 1.25 mL)

 95050-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa qPCR SuperMix
2000 x 50 μL rxns (1 x 50 mL)

 95050-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa qPCR SuperMix ROX
500 x 50 μL rxns (10 x 1.25 mL)

 95051-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa qPCR SuperMix ROX
2000 x 50 μL rxns (1 x 50 mL)

 95051-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa qPCR SuperMix Low-ROX
500 x 50 μL rxns (10 x 1.25 mL)

 95052-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

PerfeCTa qPCR SuperMix Low-ROX
2000 x 50 μL rxns (1 x 50 mL)

 95052-02K 2000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Photos

Features

PerfeCTa qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this supermix is AccuStart Taq DNA polymerase, which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95ºC), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Contents

  • PerfeCTa qPCR SuperMix (2X): 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, and stabilizers.

Storage & Handling

  • Store components in a constant temperature freezer at -25°C to -15°C upon receipt, protected from light.
  • For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Resources

Product Manuals

CofA (PSFs)

SDSs

Publications

Role of Diospyros peregrina fruit preparation in suppressing regulatory T (Treg) cells in the tumor microenvironment of breast and lung cancer
Rishabh Rai Kaushik – 2022

p53 regulates skeletal muscle mitophagy and mitochondrial quality control following denervation-induced muscle disuse
Jonathan M. Memme – 2021

Differential expression of Oct3/4 in human breast cancer and normal tissues
Feng-Qi Zhao – 2018

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