PerfeCTa Multiplex qPCR ToughMix

Features

• 1-tube SuperMix minimizes pipetting steps, simplifying reaction assembly and improving accuracy
• 5x concentrated reagent provides more flexibility with dilute DNA samples sensitivity
• ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
• Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
• Supports efficient vortex mixing with proprietary anti-foaming additives

Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with co-amplification of four abundant (106) targets. PerfeCTa MultiPlex qPCR ToughMix enables multiplex qPCR assay performance comparable to single-plex qPCR probe assays without the need to rigorously titrate primer concentration. PerfeCTa MultiPlex qPCR ToughMix is a 5x concentrated, ready to-use reaction cocktail for real-time quantitative PCR (qPCR) with ToughMix reagent technology that neutralizes a broad spectrum of PCR inhibitors that compromise assay performance with crude extracts, clinical specimens, plant, soil environmental or complex food matrices. A key component of the kit is an ultrapure, highly-processive thermostable DNA polymerase that is free of detectable E. coli DNA. PPerfeCTa MultiPlex qPCR ToughMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples.

Instrument Compatibility

ROX

• Applied Biosystems 5700
• Applied Biosystems 7000
• Applied Biosystems 7300
• Applied Biosystems 7700
• Applied Biosystems 7900
• Applied Biosystems 7900HT
• Applied Biosystems 7900 HT Fast
• Applied Biosystems StepOne™
• Applied Biosystems StepOnePlus™

Low ROX

• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Stratagene Mx3000P®
• Stratagene Mx3005P™
• Stratagene Mx4000™
• Applied Biosystems ViiA 7
• Applied Biosystems QuantStudio™ (all models)
• Douglas Scientific IntelliQube®

No ROX

• Quantabio Q
• BioRad CFX
• Roche LightCycler 480
• QIAGEN Rotor-Gene Q
• Agilent AriaMx
• Azure Cielo™
• Other

Bio-Rad iCycler iQ systems

• BioRad iCycler iQ™
• BioRad MyiQ™
• BioRad iQ™5

Contents
Single-tube, 5X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
• AccuStart II Taq DNA Polymerase.
• Proprietary enzyme stabilizers and performance-enhancing additives.
• Titrated reference dye (if applicable).

Storage & Handling
Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not impair product performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

PerfeCTa Multiplex qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.