NxGen T7 RNA Polymerase

Reliable and robust enzyme for production of mRNA transcripts in a variety of applications.

Key features

  • Reliable transcription
Name SKU Size Availability Vendor Price Order
NxGen T7 RNA Polymerase 30223-1 25,000 U @ 50 U/µL Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Features

Product information

T7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.

Product Details

Product information
T7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.

Product specifications and usage

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37 °C.

Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.

Unit Concentration 50,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease 500 U <0.1% released
DS Exonuclease 500 U <0.1% released
Endonuclease 500 U <10% converted
RNase Contamination 500 U = none detected
E. coli 16S rDNA Contamination 500 U <10 copies
Storage -20 °C

Resources

SDS

Manuals and User Guides

Product information sheets

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