Mix-n-Stain™ STORM CF® Dye Antibody Labelling Kits

Label your antibody with one of our CF® Dyes specialized for STORM super-resolution microscopy. Labelling takes just 30 minutes without a purification step, and yields an antibody conjugate with a low degree of labelling optimal for STORM.

Name SKU Size Availability Vendor Price Order

Mix-n-Stain™ STORM CF®505 Antibody Labelling Kit, 1x50ug labelling (505/519 Ex/Em nm)

 92549 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®568 Antibody Labelling Kit, 1x50ug labelling (562/584 Ex/Em nm)

 92550 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®583R Antibody Labelling Kit, 1x50ug labelling, (585/609 Ex/Em nm)

 92551 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®597R Antibody Labelling Kit, 1x50ug labelling, 597/619 Ex/Em nm

 92553 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®647 Antibody Labelling Kit, 1x50ug labelling, (652/668 Ex/Em nm)

 92554 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®660C Antibody Labelling Kit, 1x50ug labelling, (667/685 Ex/Em nm)

 92555 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®680 Antibody Labelling Kit, 1x50ug labelling, (681/698 Ex/Em nm)

 92556 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ STORM CF®750 Antibody Labelling Kit, 1x50ug labelling, (755/779 Ex/Em nm)

 92557 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Photos

Features

Mix-n-Stain™ STORM CF® Dye Antibody Labelling Kits allow you to label 50 ug of your antibody with one of Biotium’s STORM CF® Dyes to produce an antibody conjugate with a low 1-2.5 DOL (degree of labelling) optimal for super-resolution STORM (Stochastic Optical Reconstruction Microscopy). Labelling takes just 30 minutes, with minimal hands-on time and no purification.

Features

  • Label 50 ug antibody
  • Choice of 8 CF® Dye colours ideal for STORM
  • CF®583R and CF®597R are novel green-excited STORM dyes
  • Labeling optimized to provide low 1-2.5 DOL
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification, 100% recovery

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive STORM CF® Dye
  • Mix-n-Stain™ Storage Buffer

Superior CF® Dyes for STORM Imaging
These kits feature a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits are designed to offer a low degree of labeling (DOL, or dye molecules per antibody molecule) of 1-2.5, which is reported to be optimal for STORM (1). Learn more about CF® Dyes for STORM or our other fluorescent reagents validated for super-resolution microscopy.

Discover More with Green-Excitable CF® Dyes Developed for Multi-Colour STORM
High-quality STORM imaging relies heavily on bright fluorescent dyes with excellent photoswitching properties. While there are several commonly used red-excited dyes suitable for STORM imaging such as CF®647, CF®660C and CF®680, generating comparable dyes for other excitation sources to produce quality multi-colour STORM images remains a challenge. Biotium scientists have met this challenge by developing novel chemical modifications for green-excited fluorescent dyes to offer optimal performance for STORM super-resolution imaging . This includes CF®583R and CF®597R which were developed specifically for STORM imaging and display exceptional performance for multi-colour STORM (2).

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye. Select the links in the Dye Features column to learn more about each CF® Dye and potential applications.

Other Labelling Kits

We now offer Mix-n-Stain™ Maxi Antibody Labelling Kits for labelling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labelling antibodies biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labelling Kits.

References
1. Proc. SPIE 9331, (2015), DOI: 10.1117/12.2083209
2. Angew. Chem. Int. Ed. 9331, e202113612, (2021), DOI: 10.1002/ANIE.202113612

Download a list of Mix-n-Stain™ references.
Download a list of CF® Dye references.

Resources

Protocol:

SDS:

Supporting Documents:

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