Mix-n-Stain™ CF® Dye IgM Antibody Labelling Kits

Label your IgM antibody with one of Biotium’s superior CF® Dyes in 15-30 minutes without a purification step.

Name SKU Size Availability Vendor Price Order

Mix-n-Stain™ CF®405M IgM Antibody Labeling Kits, 1x25ug labeling

92560 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®405M IgM Antibody Labeling Kits, 1x100ug labeling

92561 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®594 IgM Antibody Labeling Kits, 1x25ug labeling

92568 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®594 IgM Antibody Labeling Kits, 1x100ug labeling

92569 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®488A IgM Antibody Labeling Kits, 1x25ug labeling

92562 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®488A IgM Antibody Labeling Kits, 1x100ug labeling

92563 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®555 IgM Antibody Labeling Kits, 1x25ug labeling

92564 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®555 IgM Antibody Labeling Kits, 1x100ug labeling

92565 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stai™ CF®568 IgM Antibody Labeling Kits, 1x25ug labeling

92566 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®568 IgM Antibody Labeling Kits, 1x100ug labeling

92567 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®594 IgM Antibody Labeling Kits, 1x25ug labeling

25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®594 IgM Antibody Labeling Kits, 1x100ug labeling

100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®640R IgM Antibody Labeling Kits, 1x25ug labeling

92570 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®640R IgM Antibody Labeling Kits, 1x100ug labeling

92571 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®647 IgM Antibody Labeling Kits, 1x25ug labeling

92572 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF®647 IgM Antibody Labeling Kits, 1x100ug labeling

92573 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ FITC IgM Antibody Labeling Kits, 1x25ug labeling

92574 25 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ FITC IgM Antibody Labeling Kits, 1x100ug labeling

92575 100 ug labeling Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits allow you to label 25 ug or 100 ug of your IgM antibody in 15-30 minutes, with minimal hands-on time and no purification.

Features Kit Components
  • Label 25 ug or 100 ug IgM antibody
  • Choice of 8 CF® Dye colors and FITC
  • Less than 30 seconds of hands-on time
  • 15-30 minute total reaction time
  • No purification, 100% recovery
  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ IgM Storage Buffer

Finally! Simple & Efficient Labeling of IgM Antibodies
IgM antibodies are difficult to label with fluorescent dyes because the conditions typically used for labeling IgG generally do not result in bright conjugates with good antibody reactivity for IgM. Biotium has resolved this issue by optimizing the reaction conditions for labeling IgM antibodies with Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Simply mix your purified IgM antibody with the reaction buffer and pre-measured dye provided, followed by a brief incubation. Any free dye or label in solution is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. The resulting conjugate is produced with optimal degree of labeling (DOL) for cell staining. Mix-n-Stain™ labeling is covalent, therefore labeled antibodies can be used for multiplex staining without the transfer of dye between proteins.

Superior CF® Dyes
These kits feature a choice of 8 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choose the Right Labeling Kit for Your Antibody
Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits are very simple to use. But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the Product Protocol to help you determine if your IgM antibody is compatible with the labeling protocol, or if it requires further purification.

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.

Other Labeling Kits

Check out our main line of Mix-n-Stain™ CF® Dye Antibody Labeling Kits and Mix-n-Stain™ Maxi Antibody Labeling Kits designed for rapid and efficient labeling of IgG antibodies. We also offer Mix-n-Stain™ Nanobody Labeling Kits for labeling single-domain Nanobodies®. Mix-n-Stain™ kits also are available for labeling antibodies with biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits – Biotium

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits


Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

Antibody & Protein Labeling Kits



Nanobody is a registered trademark of Ablynx, a Sanofi company.

REFERENCES

Download a list of curated CF® Dye references.

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