MC1061 F-Electrocompetent Cells

MC1061 F- Electrocompetent Cells, 12 Reactions (DUOs)

High efficiency F-control strain for phage display applications.

Key features

  • Perfect for antibody phage display or peptide phage display library creation
  • The highest efficiency TG1 competent cells available
  • Your only source for electrocompetent SS320 and ER2738 cells
  • Amber suppressor and non-amber suppressor lines for maximum flexibility
  • High efficiency MC1061 F- cells for cloning or control reactions
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells
Name SKU Size Availability Vendor Price Order

MC1061 F- Electrocompetent Cells, 12 Reactions (DUOs)

 60514-1 12 reactions Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

MC1061 F- Electrocompetent Cells, 24 Reactions (DUOs)

 60514-2 24 reactions Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Features

Product Details

Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Product information

MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 10¹⁰ cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.

Transformation efficiency

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor’s specification.

Table 1. Competent Cells for Phage Display libraries

Cell Lines Transformation Efficiency
(cfu/µg pUC DNA)		 Cloning Methylated DNA BAC, Cosmid Cloning Blue/White Screening
TG1 Electrocompetent ≥4 × 10¹⁰ YES NO YES IPTG induction required
SS320 (MC1061 F’) Electrocompetent ≥4 × 10¹⁰ YES NO YES IPTG induction required
MC1061 F-Electrocompetent ≥4 × 10¹⁰ YES NO YES IPTG induction required
ER2738 Electrocompetent ≥2 × 10¹⁰ YES NO YES IPTG induction required

Product specifications and usage

Genotype

  • TG1: [F’ traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK – mK -)
  • SS320 (MC1061F’): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
  • ER2738: [F’proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
  • MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1

Resources

SDS

Manuals and User Guides

Product information sheets

Application notes

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