Name: Geneaid Blood/Cell RNA Mini Kit with DNase
SKU: 13578
Availability: BackOrder

RNA Extraction/Total RNA

The RNA Kit (Blood/Cultured Cell) is a RNA Isolation Kit designed for total RNA purification from fresh whole human blood and cultured cells. This RNA Kit will purify RNA from blood within 20 minutes using an efficient RNA miniprep system. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and ready for use in various downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.

Name	SKU	Size	Availability	Vendor	Price	Order
Total RNA Mini Kit (Blood/Cultured Cell)	RBD050	50 Rxns	Generally 1-2 weeks from receipt of order	Geneaid			Log in for pricing
Total RNA Mini Kit (Blood/Cultured Cell)	RBD100	100 Rxns	Generally 1-2 weeks from receipt of order	Geneaid			Log in for pricing
Total RNA Mini Kit (Blood/Cultured Cell)	RBD300	300 Rxns	Generally 1-2 weeks from receipt of order	Geneaid			Log in for pricing

Specifications

Purify total RNA within 20 minutes!
High Yield: up to 2 µg from whole blood, up to 30 µg from 1×106 293T cells
Sample Volume: up to 300 µl of whole blood, cultured cells (up to 5 x 106)
Spin Columns: glass fiber membrane optimized for total RNA extraction
Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C

Applications

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis

Components

RBC Lysis Buffer
RB Buffer
DNase I
DNase I Reaction Buffer
W1 Buffer
Wash Buffer
RNase-free Water
RB Columns
2 ml Collection Tubes

Quality Control

The quality of the Total RNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Total RNA from 300 μl of fresh whole human blood is extracted using the Total RNA Mini Kit (Blood/Cultured Cell). The purified RNA (2 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.