Firefly Luciferase Assay Kit (Lyophilized)

Features

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

Features

• Highly sensitive & linear flash-type assay
• Substrate is added fresh every assay, for consistent signal over time
• Lyophilized buffer for economical shipping and convenient storage
• Excellent performance & great value compared to other suppliers

Kit Components

• 5X Firefly Luciferase Lysis Buffer
• D-Luciferin
• Firefly Luciferase Assay Buffer (Lyophilized)

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission. Firefly luciferase follows Michaelis-Menten kinetics and, as a result, maximum light output is not achieved until the substrate and co-factors are present in large excess. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

Note: This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. It requires a single tube luminometer or luminescence microplate reader with reagent injectors. Biotium also offers the Steady-Luc HTS Firefly Assay Kit (cat. no. 30028L), which is a homogenous glow-type assay with signal half-life of 3-5 hours.

Easy-to-Use for Consistent Results
This firefly luciferase assay kit is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. The kit performs comparably to Biotium’s Firefly Luciferase Assay Kit 2.0, but includes assay buffer in lyophilized format for more economical shipping and convenient storage at -20°C.

Other commercial luciferase assays require you to dilute all of the firefly substrate in the assay buffer, resulting in limited shelf life after first use. In Biotium’s kits, the substrates are provided as separate stock solutions that are freshly diluted for every assay, for excellent shelf life with consistent signal over time.

Assay size is based on 96-well format. The kits include enough cell lysis buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. Additional 5X Firefly Lysis Buffer (catalog no. 99923) can be purchased separately if needed.

Biotium also offers the Firefly & Renilla Luciferase Single Tube Assay Kit (catalog no. 30081), a combined luciferase assay kit for sequential measurement of Firefly and Renilla luciferase activity sequentially in the same sample with high sensitivity and linearity.