EpiScript™ RNase H Reverse Transcriptase Kit

Features

EpiScript RNase H- Reverse Transcriptase Kit, 200 U/µL, 10,000 U

Key Features:

• Produce full-length cDNA with RNAse H-mutant
• Active at elevated temperatures up to 55 °C

Product Details
Kit Contents: EpiScript RNase H- Reverse Transcriptase (200 U/µL), 10X Reaction Buffer, 100 mM DTT.

EpiScript RNase H– Reverse Transcriptase (EpiScript RT), is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.

Applications
First-strand cDNA synthesis for subsequent PCR or real-time PCR.

Benefits
Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity
Active at temperatures up to 55 °C
Highly efficient at producing full-length cDNAs

Product specifications and usage
Storage: Store only at -20 °C in a freezer without a defrost cycle.

Storage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.

Unit Definition: One unit of EpiScript RT catalyses the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37 °C using saturating amounts of oligo(dT)-primed poly(rA) as template.

Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.