Name: Endonuclease VIII
SKU: 2659
Availability: BackOrder

Endonuclease VIII is a DNA repair enzyme that functions as both an N-glycosylase and an AP lyase, leaving terminal phosphates at the 5′ and 3′ ends

Key features

• Removes oxidatively damaged pyrimidines from DNA

• Has both β and δ lyase activity, whereas Endonuclease III only has β lyase activity

Name	SKU	Size	Availability	Vendor	Price	Order
Endonuclease VIII 1,000 Units at 10 U/μL	30270	1,000 U @ 10 U/μl	Generally 1-2 weeks from receipt of order	LGC Biosearch Technologies - Lucigen (Epicentre)			Log in for pricing
Endonuclease VIII 10,000 Units at 10 U/μL	30271	10,000 U @ 10 U/µL	Generally 1-2 weeks from receipt of order	LGC Biosearch Technologies - Lucigen (Epicentre)			Log in for pricing

Features

Product information

E.coli Endonuclease VIII is a bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA generating an apurinic (AP) site. The AP lyase activity cleaves the DNA phosphodiester backbone at AP sites via β- and δ-elimination, creating a one nucleotide gap with 5′ and 3′ phosphate termini. Damaged bases recognised and removed by Endonuclease VIII include thymine glycol, uracil glycol, urea, 5,6-dihydroxythymine, 5-hydroxy-5-methylhydantoin, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil and alloxan.

Product Details

Endonuclease VIII Kit contains Endonuclease VIII enzyme (10 U/µL) and 10X Reaction Buffer.