E. cloni® 5-alpha Chemically Competent Cells

  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.).
  • Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
Name SKU Size Availability Vendor Price Order

E. cloni® 5-alpha Chemically Competent Cells, 12 rxns (DUOs)

 60602-1 12 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

E. cloni® 5-alpha Chemically Competent Cells, 24 rxns (DUOs)

 60602-2 24 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols.  E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.

E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

Custom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing!

Table 1. E. cloni Competent Cells for Cloning

E. cloni Cell Lines Transformation Efficiency
(cfu/µg pUC DNA)		 Cloning Methylated DNA BAC, Cosmid Cloning Blue/White Screening
5-alpha Chemically Competent >1 × 108 cfu/µg DNA NO NO YES
No IPTG induction required

 

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