E. cloni® 5-alpha Chemically Competent Cells

E. cloni® 5-alpha Chemically Competent Cells

Clone and subclone with high efficiency, chemically competent DH5-alpha cells.

Key features

  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.).
  • Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
Name SKU Size Availability Vendor Price Order

E. cloni® 5-alpha Chemically Competent Cells, 12 rxns (DUOs)

 60602-1 12 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

E. cloni® 5-alpha Chemically Competent Cells, 24 rxns (DUOs)

 60602-2 24 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Product Details

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Product Information

E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.

E. cloni 5-alpha Chemically Competent Cells >1 × 10 8 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

Table 1. E. cloni Competent Cells for Cloning

Product specifications and usage

Genotype

E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Efficiencies

E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA

Resources

Manuals and user guides

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