E. cloni® 10G ELITE Electrocompetent Cells DUOs

E. cloni® 10G ELITE Electrocompetent Cells SixPacks DUOs

Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.

  • Direct replacements for standard cloning strains
  • Optimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
  • Available in a range of high transformation efficiencies.
  • Convenient packaging options
  • High efficiency cells result in high colony counts
Name SKU Size Availability Vendor Price Order

E. cloni® 10G ELITE Electrocompetent Cells, 12 rxns (DUOs)

 60052-1 12 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

E. cloni® 10G ELITE Electrocompetent Cells, 24 rxns (DUOs)

 60052-2 24 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Product Details

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Choice of strain:

  • E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
  • E. cloni 10GF′Competent Cells. Contain the F’ plasmid for infection with M13 to produce ssDNA.

 

Choice of efficiency:

  • E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
    SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
  • E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
    ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
  • E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
    CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.

 

Custom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.

Plasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)

DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells

 Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)

Figure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).

Figure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)

Table 1. E. cloni Competent Cells for Cloning and Library Construction

E. cloni Cell Lines Transformation Efficiency
(cfu/µg pUC DNA)		 Cloning Methylated DNA BAC, Cosmid Cloning Blue/White Screening
10G SUPREME Electrocompetent >4 × 1010 YES YES YES
without IPTG induction
10G ELITE Electrocompetent >2 × 1010 YES NO YES
without IPTG induction
10G CLASSIC Electrocompetent >5 × 109 YES NO YES
without IPTG induction
10GF´ ELITE Electrocompetent >2 × 1010 YES NO YES
IPTG induction required

Efficiencies

  • E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
  • E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
  • E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA

Resources

SDS

Manuals & User Guides

Product Information Sheets

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