Novel lipophilic fluorescent dyes for selective staining of the plasma membrane in formaldehyde-fixed cells. Available in 6 colors, from blue to near-IR.
| Name | SKU | Size | Availability | Vendor | Price | Order | |
CytoLiner™ 680/710 Fixed Cell Membrane Stain, 250 labelings |
30135-T | 250 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 680/710 Fixed Cell Membrane Stain, 1000 labelings |
30135 | 1000 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 650/675 Fixed Cell Membrane Stain, 250 labelings |
30134-T | 250 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 650/675 Fixed Cell Membrane Stain, 1000 labelings |
30134 | 1000 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 570/590 Fixed Cell Membrane Stain, 250 labelings |
30133-T | 250 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 570/590 Fixed Cell Membrane Stain, 1000 labelings |
30133 | 1000 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 495/510 Fixed Cell Membrane Stain, 250 labelings |
30132-T | 250 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 495/510 Fixed Cell Membrane Stain, 1000 labelings |
30132 | 1000 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 410/450 Fixed Cell Membrane Stain, 250 labelings |
30131-T | 250 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing | ||
CytoLiner™ 410/450 Fixed Cell Membrane Stain, 1000 labelings |
30131 | 1000 labelings | Generally 1-2 weeks from receipt of order | Biotium | Log in for pricing |
CytoLiner™ Fixed Cell Membrane Dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.
| Features | Kit components |
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Novel Membrane Dyes Designed for Fixed Cells
While classic lipophilic membrane dyes like DiI can be used to stain formaldehyde-fixed cells, staining can be highly variable due to the poor solubility of the dyes. CytoLiner™ Dyes are a new generation of membrane dyes uniquely engineered to permit selective staining of the plasma membrane in fixed and mildly permeabilized cells. CytoLiner™ staining is compatible with formaldehyde fixation and mild detergent permeabilization before staining. Staining can tolerate blocking agents used during immunofluorescence staining protocols, allowing subsequent staining with antibodies and other probes. The dyes also are compatible with poly-L-lysine coated cultureware and Transwell® permeable filter supports. CytoLiner™ Dyes are available in a selection of 6 colors from blue to near-IR.
| Superior Staining Performance Over Classic Membrane Dyes |
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| Comparison of fixed cell staining with CytoLiner™ Fixed Cell Membrane Stains versus classic lipophilic carbocyanine membrane dyes. In comparison to the traditional lipophilic carbocyanine dyes, CytoLiner™ dyes show more uniform and reliable staining of PFA-fixed, mildly-permeabilized cell membranes. PFA-fixed MCF7 cells were permeabilized for 10 minutes at RT with PBS/0.1% Triton® X-100, then rinsed with PBS and stained with carbocyanine dyes in PBS or CytoLiner™ Stains in 1X CytoLiner™ Buffer for 10 minutes at RT, then rinsed with PBS. Imaged by confocal microscopy; scale bar: 20 um. |
| Suitable for Co-Staining with Antibodies and Other Probes |
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| PFA-fixed MCF-7 cells were permeabilized with PBS/0.1% Triton® X-100 for 10 minutes at room temperature, then stained with 1X CytoLiner™ Fixed Cell Membrane Dyes and NucSpot® Nuclear Stains. |
Find the Right Stain for Your Application
CytoLiner™ Dyes are part of Biotium’s family of unique and innovative cell membrane stains designed for specific applications. For workflows requiring staining live cells before fixation, we recommend our CellBrite® Fix Membrane Stains or MemBrite® Fix Cell Surface Staining Kits. Biotium also offers CF® Dye conjugated lectins, including Concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), for visualizing cell surfaces in live or fixed cells. For longer term monitoring of cell surfaces in live cells, we recommend CellBrite® Steady Dyes. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure.
Watch our video where Technical Applications Scientist II, Jacqueline Steenhuis PhD answers your top questions about Biotium’s various membrane stains for fluorescence microscopy.
MemBrite® Fix Cell Surface Staining Kits – Biotium
For additional support or product recommendations, contact us at info@genetargetsolutions.com.
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