Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase

Cheetah™ PEA-PLEX HotStart Taq is a unique formulation of Biotium’s chemically modified Cheetah™ Taq polymerase validated by third parties specifically for NGS-based high-throughput proximity extension assays (PEA) for protein detection. Cheetah™ PEA-PLEX is suitable for fast cycling qPCR, as well.

Features

  • Unique formulation for NGS-based high-throughput PEA
  • Also works for fast cycling qPCR
  • Rapid hot-start activation via novel chemical modification
  • Antibody-free, low-DNA Taq for cleaner results
  • Superior long-term stability compared to other chemically modified Taq
  • Bulk formats available
Name SKU Size Availability Vendor Price Order

Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase 100 uL (500 U)

 29138-100UL 100 uL Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase 1 mL (5 kU)

 29138-1ML 1 mL Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Uniquely formulated for NGS-based high-throughput PEA

Cheetah™ PEA-PLEX HotStart Taq is a highly optimized formulation that was developed specifically for NGS-based high-throughput proximity extension assays (PEA). Similar to Cheetah™ FLEX HotStart Taq, Cheetah™ PEA-PLEX HotStart Taq features a novel chemical modification that completely suppresses its activity prior to activation, and offers a much faster activation time than AmpliTaq Gold® or HotStartTaq®.

Antibody-based hot-start Taq polymerases, such as AmpliTaq Gold®, can suffer from inconsistent activation, limited pH tolerance, reduced shelf life, and contamination risk from animal DNA. Cheetah™ PEA-PLEX HotStart Taq solves these problems with a proprietary chemical modification that ensures complete inactivity until heat activation—no antibodies, no animal DNA. It offers a rapid 2-minute activation at 95°C—up to 10x faster than AmpliTaq Gold® or HotStarTaq®. Cheetah™ PEA-PLEX is also more stable due to reduced risk of intramolecular cross-linking that can occur in AmpliTaq® Gold. It delivers the same fidelity and efficiency as native Taq polymerase.

Cheetah™ FLEX HotStart Taq offers a flexible formulation suitable for both fast cycling PCR and non-NGS PEA assays. Also explore our Forget-Me-Not™ qPCR Master Mixes featuring Cheetah™ Taq.

Cheetah-Taq-Hot-Start
Cheetah™ Taq requires only 2 minutes of heat activation for full recovery of activity. Comparison of hot-start recovery of polymerase activity for Cheetah™ Taq and AmpliTaq Gold® following incubation at 95°C in 50 mM pH 8.0 Tris.

AmpliTaq Gold is a registered trademark of Thermo Fisher Scientific; HotStarTaq is a registered trademark of Qiagen.

Resources

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SDS

Supporting Documents

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