Cheetah™ FLEX HotStart Taq DNA Polymerase

Cheetah™ FLEX HotStart Taq is Biotium’s proprietary chemically modified Taq polymerase designed for reducing non-specific DNA amplification in PCR. Cheetah™ Taq features a novel chemical modification that completely suppresses its activity prior to activation, and offers a much faster activation time than AmpliTaq Gold® or HotStartTaq®. Cheetah™ FLEX HotStart Taq is validated for fast cycling PCR and proximity extension assays (PEA).

Features

  • Rapid hot-start activation via novel chemical modification
  • Validated for fast cycling PCR and PEA assays
  • Antibody-free, low-DNA Taq for cleaner results
  • Superior long-term stability compared to other chemically modified Taq
  • Convenient ready-to-use kit or bulk formats available

Components (29079-KIT Only)

  • Cheetah™ Taq, 5 U/uL, 100 uL
    (in buffer containing Tris-HCl pH 9.0, DTT, EDTA, KCl and glycerol)
  • 10X Cheetah™ Taq Dilution Buffer (without Mg2+), 1.5 mL
  • 25 mM MgCl2, 1.5 mL
Name SKU Size Availability Vendor Price Order

Cheetah™ FLEX HotStart Taq DNA Polymerase – 1 kit, 500 U Taq with Buffer Pack

 29079-KIT 1 Kit Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Cheetah™ FLEX HotStart Taq DNA Polymerase – 1 mL (5 kU), Enzyme Only

 29079-1ML 1 mL Generally 1-2 weeks from receipt of order Biotium Log in for pricing

A superior HotStart Taq for FLEXible applications

Antibody-based hot-start Taq polymerases, such as AmpliTaq Gold®, can suffer from inconsistent activation, limited pH tolerance, reduced shelf life, and contamination risk from animal DNA. Cheetah™ FLEX HotStart Taq solves these problems with a proprietary chemical modification that ensures complete inactivity until heat activation—no antibodies, no animal DNA. It offers a rapid 2-minute activation at 95°C—up to 10x faster than AmpliTaq Gold® or HotStarTaq®. Cheetah™ FLEX is also more stable due to reduced risk of intramolecular cross-linking that can occur in AmpliTaq® Gold. It delivers the same fidelity and efficiency as native Taq polymerase.

Cheetah™ FLEX HotStart Taq is validated for fast-cycling PCR and also third-party validated for qPCR-based proximity extension assay (PEA) applications for protein detection. Cheetah™ FLEX HotStart Taq is available as a ready-to-use kit or standalone enzyme.

Also explore our Forget-Me-Not™ qPCR Master Mixes featuring Cheetah™ Taq, and Cheetah™ PEA-PLEX, specially formulated for NGS-based, high-throughput PEA.

Cheetah-Taq-Hot-Start
Cheetah™ Taq requires only 2 minutes of heat activation for full recovery of activity. Comparison of hot-start recovery of polymerase activity for Cheetah™ Taq and AmpliTaq Gold® following incubation at 95°C in 50 mM pH 8.0 Tris.

AmpliTaq Gold is a registered trademark of Thermo Fisher Scientific; HotStarTaq is a registered trademark of Qiagen.

Resources

Protocols

SDS

Supporting Documents

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