- Cells that enable the most challenging library construction and screening.
- Highest available transformation efficiencies for library construction and screening.
- Optimized for high yields of DNA.
| Name | SKU | Size | Availability | Vendor | Price | Order | |
E. cloni® 10G BAC-Optimized Electrocomp Cells,25 rxns (FIVERs) |
60215-2 | 25 Rxns | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing | ||
Recovery Medium, 8 x 12 mL |
80026-1 | 8 x 12 mL | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing | ||
AmpliScribe™ T7 High Yield Transcription Kit |
60210-1 | 12 Rxns | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing | ||
BAC-Optimized Replicator™ v2.0 Electrocomp Cell,24 rxns (DUOs) |
60210-2 | 24 Rxns | Generally 1-2 weeks from receipt of order | LGC Biosearch Technologies - Lucigen (Epicentre) | Log in for pricing |
Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:
BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight® Cloning Kits.
E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
| E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G BAC-Optimized Electrocompetent |
>1 × 1010 |
YES |
YES |
YES |
|
BAC-Optimized Replicator v2.0 Electrocompetent |
>1 × 1010 |
YES |
YES |
YES |
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E. cloni® 10G Chemically Competent Cells DUOs
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E. cloni® 10G CLASSIC Electrocompetent Cells SixPacks
Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.). Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
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E. cloni® 10G ELITE Electrocompetent Cells SixPacks
Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.). Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
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E. cloni® EXPRESS BL21(DE3) Competent Cells
Best value for routine protein expression… available as Electrocompetent and Chemically Competent Cells.
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