Annexin V Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Name SKU Size Availability Vendor Price Order

Annexin V, CF®350 Conjugate 50ug/mL 347/448nm

 29012 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®405M Conjugate 50ug/mL 408/452nm

 29009 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®450 Conjugate 50ug/mL 450/538nm

 29083 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®488A Conjugate 50ug/mL 490/515nm

 29005 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®555 Conjugate 50ug/mL 555/565nm

 29004 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®568 Conjugate 50ug/mL 562/583nm

 29010 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®583R Conjugate 50ug/mL 586/609nm

 29085 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®594 Conjugate 50ug/mL 593/614nm

 29011 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®633 Conjugate 50ug/mL 630/650nm

 29008 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®640R Conjugate 50ug/mL 642/662nm

 29014 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®647 Conjugate 50ug/mL 650/665nm

 29003 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®660R Conjugate 50ug/mL 663/682nm

 29069 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, CF®740 Conjugate 50ug/mL 742/767nm

 29126 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, R-phycoerythrin Conjugate (20 assays) 496, 546, 565/578nm
5uL test

 29045-100uL 100ul Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, R-phycoerythrin Conjugate (100 assays) 496, 546, 565/578nm
5uL test

 29045-500uL 500uL Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, Allophycocyanin Crosslinked Conjugate (20 assays), 650/660nm
5uL/test

 29057-100uL 100ul Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, Allophycocyanin Crosslinked Conjugate (100 assays), 650/660nm
5uL/test

 29057-500uL 500uL Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, FITC Conjugate 50ug/mL 496/516nm

29001 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Annexin V, Biotin Conjugate 50ug/mL

 29013 0.5ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

5X Annexin V Binding Buffer

 99902 15ml Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Photos

Features

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

  • Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalization
  • Conjugates with wide choice of CF® Dye colors, plus biotin, R-PE, APC, and other fluorescent dyes
  • Biotium’s CF® Dyes have superior brightness, photostability, and are water-soluble
  • Can be used for fluorescence microscopy, live cell imaging, and flow cytometry

CF® Dye, biotin, and FITC conjugates of Annexin V are provided as 50 ug/mL solutions in 10 mM Tris, 1 mM EDTA, 30 mM NaCl, 1 mg/mL bovine serum albumin, 0.1% sodium azide, pH 7.5. R-PE and APC Annexin V conjugates are supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide at 5 uL per test.

Detect Apoptosis
We also offer Annexin V Conjugates, Azide-Free and Lyophilized in colours ranging from UV to far-red for live cell staining, and Near-IR CF® Dye Annexin V (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See Biotium’s full selection of Cell Viability and Apoptosis Assays.

Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colours.

Resources

Protocols:

SDS:

Supporting Documents:

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